Fig 2: The relative expression levels of nine key oxidation-related genes. (A). INOS; (B). HO-1; (C). CAT; (D). Nrf2; (E). Keap1; (F). PGC1a; (G). SOD1; (H). SOD2; (I). SOD3; Means ± SD; n = 6; *p < .05, compared with the model group; **p < .01, compared with the model group; ## p < .05, compared with the control group.

Fig 3: IHC analysis of the testis tissues. Immunopositive expression of Nrf2, Keap1, SOD2, SOD3, CDC25C, and CDK1 was observed. Original magnification, ×200.

Fig 4: Effects of amentoflavone on the expression of Nrf2/HO-1 levels in LPS-treated BV2 microglial cells. (a–c) Semiquantitative analysis of the relative levels of MDA, GSH, and SOD. (d) Representative immunoblots of Keap1, Nrf2, and HO-1 proteins in BV2 microglial cells. (e) BV2 microglial cells were treated with amentoflavone (10 µ?) or 0.5% DMSO for 1 h, followed by treatment with LPS (1 µg/ml) or PBS for 6 h. Semiquantitative analysis of the relative levels of Keap1, Nrf2, and HO-1 by densitometric analysis. Error bars represent mean ± s.d. *** represent P < 0.001, respectively. All experiments were performed in triplicate. LPS: lipopolysaccharide; AF: amentoflavone.