Fig 1: GLUT3 is key downstream of low expression of RRAD.A, B Transfection efficiency was detected using PCR and Western blot after RRAD-specific shRNA transfection in HN4 cells. ***P < 0.001. C, D Representative images of tumors derived from the xenograft model were shown. E Tumor weight was measured after excision from mice. **P < 0.01. F The tumor volume was measured and analyzed once a week. *P < 0.05, **P < 0.01. G IHC staining for RRAD was conducted on xenograft tumors. Scale bar: 200 μm. H The heatmap of DEGs of si-Scr vs. si-RRAD. I The GO enrichment based on DEGs of si-Scr vs. si-RRAD. J The relative SLC2A1, SLC2A2, SLC2A3, and SLC2A4 mRNA levels of HN4 and CAL27 cell lines. **P < 0.01. K GLUT1, GLUT2, GLUT3, and GLUT4 were detected after silencing RRAD in HN4 and CAL27 cell lines. L Survival analysis was performed in the OSCC dataset from the TCGA database. The correlation between SLC2A1, SLC2A2, SLC2A3, and SLC2A4 expression and overall survival.