Fig 1: miR-223-3p overexpression suppresses LIF expression. (A) miR-223-3p expression in the endometrium on the day of embryo transfer. *P<0.05. (B) miR-223-3p expression in ESCs treated with miR-223-3p mimics or hsa-miR negative controls. Transfection with miR-223-3p mimics significantly increased the expression of miR-223-3p. (C) LIF mRNA expression in ESCs treated with hsa-miR-223-3p mimics or negative control. (D) LIF protein expression levels following treatment with miR-223-3p mimics or negative controls were analyzed via western blotting. *P<0.05 vs. control. 1, 2 and 3 refer to the number of cycles of GnRHa, E2 and P4 treatment; miR, microRNA; LIF, leukemia inhibitory factor; ESC, endometrial stromal cell; ESC, endometrial stromal cell; mNC, modified natural cycle; HT, hormone therapy cycle; GnRHa, gonadotropin-releasing hormone agonist.