ZO-1 Polyclonal Antibody bs-1329R from Bioss Inc.

Product Specs
ItemZO-1 Polyclonal Antibody
CompanyBioss Inc.
Price $375.00Supplier Page View Company Product Page
Catalog Numberbs-1329R
Molecular Weight191kDa
NCBI Gene AliasesZO-1
ImmunogenKLH conjugated peptide, human ZO-1
Format0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol
Conjugate/TagUnconjugated
IsotypeIgG
Antibody TypePrimary Antibody
Quantity100ul, 20uL
ClonalityPolyclonal
ApplicationsWB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), WB, WB, IF(ICC), WB, IHC-P, WB, IF, IHC, IHC-P, FCM, IF
ReactivityRat, Pig, Mouse, Human, Bovine
HostRabbit
TargetZO-1
Concentration1ug/ul
NCBI Full Gene Nametight junction protein 1
Tissueheart, Mid-jejunum fragments, colon, brain

Questions and Answers

What applications has the ZO-1 Polyclonal Antibody been used for in scientific research?

The ZO-1 Polyclonal Antibody has been shown to work in various applications including Western Blotting, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry, as evidenced by its use in studies investigating intestinal barrier function and tight junction integrity in different models of disease, such as colitis and hyperuricemia.

Can this antibody be used to study tight junctions in intestinal tissues?

Yes, the ZO-1 Polyclonal Antibody has been utilized in immunostaining of intestinal tissues to assess tight junction integrity. For instance, it was employed in a study examining intestinal permeability in a mouse model of hyperuricemia, where ZO-1 expression was analyzed to evaluate the effects of hyperuricemia on intestinal barrier function ().

Is the ZO-1 antibody effective in detecting changes in tight junction protein expression in response to treatments?

Yes, the ZO-1 Polyclonal Antibody has been used to detect changes in tight junction protein expression in response to various treatments. For example, it was used in a study where the antibody helped demonstrate the protective effects of Selenomethionine and Allicin on intestinal barrier integrity by showing increased expression of ZO-1 in treated cells ().

What species does the ZO-1 Polyclonal Antibody react with?

The ZO-1 Polyclonal Antibody is reactive with multiple species, including humans, mice, rats, bovines, and porcine, making it versatile for studies across different animal models and human tissues.

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(1) A comparative study of organoid-derived and cell line-derived intestinal epithelial modelsPLoS OneApril 16, 2026Melis Asal, Nanne J. Paauw, Hetty J. Bontkes, Sandra J. van Vliet, Reina E. Mebius, Susan GibbsThermo Fisher Scientific), anti-Mucin 2–1:200 (NBP2−66961, Novus) or anti-Zonula occludens1–1:100 (Bs-1329r, Bioss Antibodies)) diluted in 2% normal goat serum in PBS. After washing 3x with PBS, secondary antibody

(2) Lysosome-Featured Cell Aggregate-Released Extracellular Vesicles Regulate Iron Homeostasis and Alleviate Post-Irradiation Endothelial Ferroptosis for Mandibular RegenerationAdvanced science (Weinheim, Baden-Wurttemberg, Germany)September 1, 2025Li YY, Ma B, Lu JW, Zhang KC, Ma C, Bai SF, Li YJ, Ying SQ, Weng WZ, Zhang K, et al.1:200), anti‐connexin 43 (Merck Millipore, USA; Cat#AB1728; diluted 1:200), anti‐ZO‐1 (Bioss, China; Cat#bs‐1329R; diluted 1:200), anti‐integrin αIIb/β3 (Santa Cruz Biotechnology, USA; Cat#sc‐21783; diluted 1:200),

(3) ShenQiGan Extract Repairs Intestinal Barrier in Weaning-Stressed Piglets by Modulating Inflammatory Factors, Immunoglobulins, and Short-Chain Fatty AcidsAnimals (Basel)July 28, 2025Rongxia Guo, Chenghui Jiang, Yanlong Niu, Chun Niu, Baoxia Chen, Ziwen Yuan, Yongli Hua, Yanming Weiseparated by SDS-PAGE electrophoresis. For protein blot analysis, primary antibodies against ZO-1 (Bioss, bs-1329R, 1:1000), Occludin (Proteintech, 13409-1-AP, 1:4000), Claudin1 (Proteintech, 13050-1-AP, 1:1000), MUC2

(4) Probiotic Bifico Ameliorates Depression- and Anxiety-Like Behaviors Induced by Estrogen Deficiency via NLRP3 Inflammasome InhibitionJ Inflamm ResJune 20, 2025Xia Yu, Xiurong Yu, Yang Yang, Wei Cheng, Mingxiu Shi, Li Chen, Xiongle Zhang, Yongjun Xuab184787, 1:1000), ASC (Abcam, ab180799, 1:1000), Occludin (Abcam, ab216327, 1:1000) or ZO-1 (Bioss, bs-1329R, 1:600) overnight at 4°C in the refrigerator. Specific bands were visualized using an enhanced chemiluminescence

(5) Enhanced alleviation of the selenite-grafted soluble and nondigestive Chinese Yam polysaccharides on nonylphenol-induced cytotoxicity and barrier damage in intestinal epithelial cellsSci RepMay 23, 2025Zhen-Xing Wang, Li-Li Zhang, Xin-Huai ZhaoNovoprotein Biotech Co., Ltd. (Suzhou, China). Primary antibodies (GAPDH Bioss bs-10900R, ZO-1 Bioss bs-1329R) and goat anti-rabbit secondary antibody were all purchased from Bioss Biotechnology Co., Ltd. (Beijing,

(6) HGF Aggravated Periodontitis-Associated Gut Barrier and Microbial Dysfunction: Implications for Oral-Gut Axis RegulationBiology (Basel)May 2, 2025Zhen Chen, Yang Zhong, Lu Chen, Weijia Liu, Chuyin Lin, Yannan Chen, Xinhong WangShanghai China) was added, and anti-Occludin (cat: 27260-1-AP, Proteintech, Wuhan, China), anti-ZO-1 (cat: bs-1329R, Bioss, Beijing, China), and anti-NOD2 antibodies (cat: 20980-1-AP, Proteintech, Wuhan, China) were

(7) Covalent Grafting of Inorganic Selenium to the Water-Soluble and Nondigestive Chinese Yam Polysaccharides Causes Greater Protection of IEC-6 Cells with Acrylamide InjuryFoodsApril 29, 2025Zhen-Xing Wang, Li-Li Zhang, Xin-Huai ZhaoNovoprotein Biotech in Suzhou, China. Primary antibodies, including GAPDH (Bioss bs-10900R), ZO-1 (Bioss bs-1329R), and the goat anti-rabbit secondary antibody were purchased from Bioss Biotechnology in Beijing, China.

(8) Obesity-driven oleoylcarnitine accumulation in tumor microenvironment promotes breast cancer metastasis-like phenotypeActa Pharmaceutica Sinica BFebruary 26, 2025Chao Chen, Hongxia Zhang, Lingling Qi, Haoqi Lei, Xuefei Feng, Yingjie Chen, Yuanyuan Cheng, Defeng Pang, Jufeng Wan, Haiying Xu, et al.Cell Signaling Technology (anti-E-cadherin, cat#14472S; anti-N-cadherin, cat#13116S), Bioss (anti-ZO-1, cat#bs-1329R; anti-β-actin, cat#bs-0061R), Immunoway (anti-Fibronectin, cat#YM3137; anti-PAI-1, cat#YT3569), Affinity Biosciences

(9) Huanglian Ejiao Decoction Alleviates Ulcerative Colitis in Mice Through Regulating the Gut Microbiota and Inhibiting the Ratio of Th1 and Th2 CellsDrug Des Devel TherJanuary 17, 2025Jingyi Tang, Yingnan Hu, Jintao Fang, Weihan Zhu, Wenjun Xu, Dian Yu, Zhipeng Zheng, Qiujing Zhou, Huiying Fu, Wei Zhang(anti-IL-1β (GB11113, Servicebio, Wuhan, China), anti-GAPDH (GB11002, Servicebio, Wuhan, China), anti-ZO-1 (bs-1329R, Bioss, Woburn, MA, USA), anti-Occludin (#91131, CST, Danvers, MA, USA), anti-IL-6 (DF6087, Affinity Biosciences

(10) Ginsenoside Rg1 Alleviates Blood–Milk Barrier Disruption in Subclinical Bovine Mastitis by Regulating Oxidative Stress-Induced Excessive AutophagyAntioxidants (Basel)November 24, 2024Shanshan Yang, Zihao Fang, Hongwei Duan, Weitao Dong, Longfei Xiaotissue sections was executed by virtue of the rabbit polyclonal antibody against ZO-1 (diluted at 1:100; bs-1329R; Bioss), occludin (diluted at 1:100; bs-10011R; Bioss), claudin-1 (diluted at 1:100; bs-21509R; Bioss)

(11) Heat Stress Impairs Endometrial Function During Implantation by Regulating Autophagy in Hainan Black GoatAnimals (Basel)November 8, 2024Xiaoping Li, Yanyu Sun, Yi Min, Xinyu Wang, Diqi Yang, Hui Peng(TBST) and 10% nonfat milk for 2 h, the samples were incubated with primary antibodies: anti-TJP1 (Bioss bs-1329R, diluted 1:1000, Bioss Biotechnology Co., Ltd., Beijing, China), anti-MAP1LC3 (Sigma L7543, diluted

(12) Selenomethionine and Allicin Synergistically Mitigate Intestinal Oxidative Injury by Activating the Nrf2 PathwayToxicsSeptember 30, 2024Yongshi Liu, Xi Lv, Heling Yuan, Xiaoming Wang, Jinhu Huang, Liping WangKeap1(bs-3648R), Rabbit polyclonal antibody Occludin (bs-10011R), and Rabbit polyclonal antibody ZO-1 (bs-1329R) were purchased from Bioss (Beijing, China). HRP goat anti-rabbit IgG (AS014) was purchased from Abclonal

(13) Investigation of the relationship between inflammation and microbiota in the intestinal tissue of female and male rats fed with fructose: Modulatory role of metforminDARU Journal of Pharmaceutical SciencesJune 17, 2024Azimet Yalçın Buğdaycı, Saadet Özen Akarca Dizakar, Mürşide Ayşe Demirel, Suna Ömeroğlu, Fatma Akar, Mecit Orhan Uludağ(bs-1495R, Bioss), Claudin-1 (bs-1428R, Bioss), Claudin-2 (bs-7125R, Bioss), Zonula Occludens-1 (ZO-1) (bs-1329R, Bioss), E-Cadherin (bs-10009R, Bioss), and F-Actin (bs-1571R, Bioss) primer antibodies (1:200 dilution)

(14) Lactoferrin alleviates chronic low‑grade inflammation response in obese mice by regulating intestinal floraMolecular Medicine ReportsJune 13, 2024Wuji Wang, Jing Zhang, Yanyi Li, Si Su, Lisi Wei, Li Li, Rilebagen Huselected primary antibodies. The primary antibodies included zonula occludens-1 (ZO-1; 1:500; cat. no. bs-1329R; BIOSS), Occludin (1:500; cat. no. bs-10011R; BIOSS) and β-actin (1:10,000; cat. no. AB0035; Shanghai

(15) Gold nanoparticles exhibit anti-osteoarthritic effects via modulating interaction of the “microbiota-gut-joint” axisJournal of NanobiotechnologyApril 8, 2024Zihan Deng, Chuan Yang, Tingwen Xiang, Ce Dou, Dong Sun, Qijie Dai, Zhiguo Ling, Jianzhong Xu, Fei Luo, Yueqi ChenBSA at room temperature and then incubated with primary antibodies specific for ZO-1 (Bioss, Cat#: bs-1329R) and Occludin (Bioss, Cat#: bs-10011R) overnight at 4 °C. Next, these sections were conjugated with

(16) Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Ameliorate Aging-Induced BTB Impairment in Porcine Testes by Activating Autophagy and Inhibiting ROS/NLRP3 Inflammasomes via the AMPK/mTOR Signaling PathwayAntioxidants (Basel)January 31, 2024Yi Zhou, Jiale Yan, Limin Qiao, Jiaqin Zeng, Fuyu Cao, Xihui Sheng, Xiaolong Qi, Cheng Long, Bingying Liu, Xiangguo Wang, et al.Cell Signaling Technology), mTOR (1:1000 dilution; #2983; Cell Signaling Technology), ZO-1 (1:1000 dilution; bs-1329R; Bioss, Woburn, MA, USA), Occludin (1:1000 dilution; bs-10011R; Bioss), Claudin-11 (1:1000 dilution;

(17) Oregano essential oil modulates colonic homeostasis and intestinal barrier function in fattening bullsFront MicrobiolDecember 5, 2023Yue Ma, Jinping Shi, Li Jia, Pengjia He, Ying Wang, Xiao Zhang, Yongliang Huang, Qiang Cheng, Zhao Zhang, Youchao Dai, et al.monoclonal antibodies to analyze the distribution of colonic ZO-1 and MUC-2 expression in bulls. Anti-ZO-1 (bs-1329R; Bioss, China) and anti-MUC-2 antibodies (Clone ABT198; Immunoway, USA) were used for immunohistochemistry

(18) Curcumin Ameliorates Age-Induced Tight Junction Impaired in Porcine Sertoli Cells by Inactivating the NLRP3 Inflammasome through the AMPK/SIRT3/SOD2/mtROS Signaling PathwayOxidative medicine and cellular longevityJanuary 1, 2023Xiao L, Fang Z, Wang Q, Sheng X, Qi X, Xing K, Guo Y, Ni H, Wang X, Zhang Y.specific SC marker) together with the rabbit polyclonal antibody against ZO-1 (diluted at 1 : 100; bs-1329R; Bioss), Claudin-11 (dilution rate 1 : 100; bs-21509R; Bioss), or Occludin (diluted at 1 : 100; bs-10011R;

(19) Postbiotic muramyl dipeptide alleviates colitis via activating autophagy in intestinal epithelial cellsFrontiers in PharmacologyNovember 23, 2022Yaying You, Yongtao Xiao, Ying Lu, Jun Du, Hui Cai, Wei Cai, Weihui YanAntibody,Source,Catalog#,Application/dilution ZO-1,Bioss inc.,bs-1329R,WB(1:1000) E-cadherin,Cell Signaling Technology,3195P,WB(1:1000) beta-Actin,Cell Signaling Technology,4970S,WB(1:1000) GAPDH,Lablead,G0100,WB(1:5000) NOD2,Proteintech,66710-1-Ig,WB(1:1000) LC3A/B,Cell Signaling Technology

(20) Effects of Low-Ambient-Temperature Stimulation on Modifying the Intestinal Structure and Function of Different Pig BreedsAnimals (Basel)October 12, 2022Yanbo Guo, Ting Liu, Wenxia Li, Wanfeng Zhang, Chunbo Cai, Chang Lu, Pengfei Gao, Guoqing Cao, Bugao Li, Xiaohong Guo, et al.to the villus base. The expression of the tight junction (anti-zonula occludens 1 (ZO-1) antibodies, bs1329R; anti-occludin antibodies, bs10011R; Bioss, Beijing, China) was detected in duodenal tissue by immunohistochemistry (IHC

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ZO-1 Polyclonal Antibody from Bioss Inc.

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ZO-1 Polyclonal Antibody from Bioss Inc.

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