Fig 1: a X-ray radiographs of Manffl/flCol2Cre−Matn3+/+, Manffl/flCol2Cre−Matn3V194D/V194D and Manffl/flCol2Cre+Matn3V194D/V194D mice at 6 weeks, showing the dramatic effect of removal of MANF from cartilage of the mouse model of MED. b Western blot and densitometry measurement of intracellular GRP78 levels in Matn3V194D/V194D primary chondrocytes with and without exogenous MANF (n = 4). c Western blot and densitometry measurement of intracellular matrilin-3 levels in Matn3V194D/V194D primary chondrocytes with and without exogenous MANF (n = 4). d Immunocytochemistry for matrilin-3 showing that exogenous MANF has no effect on the intracellular retention of mutant matrilin-3 (in green) in Matn3V194D/V194D primary chondrocytes. DAPI was used as a counterstain. Scale bars 5 mm (a) and 200 μm (d) (colour figure online)