Published customer image:
Mouse anti Rat CD4 antiboy, clone OX-68 (MCA1022R) used for the developments of an ELISA curve detailing procedures to describe biotin- streptavidin ratios. Protocol is described.
Image caption:
Establishing the ratios of biotinylated protein to streptavidin-PE using an ELISA-based method. An example of streptavidin-PE conjugation strategy used to generate an avid probe from a biotinylated monomeric protein. A dilution series of biotinylated monomers was incubated against a fixed concentration of streptavidin. The minimum dilution at which no excess biotinylated monomers can be detected was determined by ELISA. ELISA was performed with or without preincubating a range of protein dilutions with 10 ng of streptavidin-PE. In the presence of streptavidin-PE, the minimum dilution at which no signal was identified (circled black) and the amount of protein required for the saturation was calculated to generate a 10x stock solution with 4 μg/mL streptavidin-PE.

From: Sharma S, Wright GJ.
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens.
.
doi: 10.3791/60803.
This image is from an open access article distributed under terms of a.

Published customer image:
Mouse anti Rat CD4 antiboy, clone OX-68 (MCA1022R) used for the developments of an ELISA curve detailing procedures to describe biotin- streptavidin ratios. Protocol is described here.
Image caption:
Establishing the ratios of biotinylated protein to streptavidin-PE using an ELISA-based method. An example of streptavidin-PE conjugation strategy used to generate an avid probe from a biotinylated monomeric protein. A dilution series of biotinylated monomers was incubated against a fixed concentration of streptavidin. The minimum dilution at which no excess biotinylated monomers can be detected was determined by ELISA. ELISA was performed with or without preincubating a range of protein dilutions with 10 ng of streptavidin-PE. In the presence of streptavidin-PE, the minimum dilution at which no signal was identified (circled black) and the amount of protein required for the saturation was calculated to generate a 10x stock solution with 4 μg/mL streptavidin-PE.

From: Sharma S, Wright GJ.
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens.
J Vis Exp. 2020 Jun 6;(160).
doi: 10.3791/60803.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Published customer image:
Mouse anti Rat CD4 antiboy, clone OX-68 (MCA1022R) used for the developments of an ELISA curve detailing procedures to describe biotin- streptavidin ratios. Protocol is described here.
Image caption:
Establishing the ratios of biotinylated protein to streptavidin-PE using an ELISA-based method. An example of streptavidin-PE conjugation strategy used to generate an avid probe from a biotinylated monomeric protein. A dilution series of biotinylated monomers was incubated against a fixed concentration of streptavidin. The minimum dilution at which no excess biotinylated monomers can be detected was determined by ELISA. ELISA was performed with or without preincubating a range of protein dilutions with 10 ng of streptavidin-PE. In the presence of streptavidin-PE, the minimum dilution at which no signal was identified (circled black) and the amount of protein required for the saturation was calculated to generate a 10x stock solution with 4 μg/mL streptavidin-PE.

From: Sharma S, Wright GJ.
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens.
J Vis Exp. 2020 Jun 6;(160).
doi: 10.3791/60803.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Published customer image:
Mouse anti Rat CD4 antibody, clone CD-68 (MCA1022R) used to evaluate CD4 expression by flow cytometry.
Image caption:
Surface expression of chimeric CD4 in PSC-macrophages. A) PSC-macrophages were tested for surface CD4 expression by flow cytometry using three different clones of anti-CD4 antibodies. Representative histogram showing CD4 surface staining with mAb clone 11830 (red/brown line, recognises both endogenous human and chimeric CD4) is shown in Figure 6E. Staining with mAb clone OKT4 (blue line, left panel, recognises endogenous human CD4 only); or with mAb clone OX-68 (orange line, right panel, recognises chimeric CD4 only). All lines are depicted compared to the matched isotype control (shaded gray). Representation of the binding site of the antibodies on human and chimeric CD4 are shown at the bottom. B) Quantification of endogenous human CD4 expression relative to the PSC-macrophages control group, using mAb OKT4 (n = 4). C) Quantification of chimeric CD4 relative to the PSC-macrophages CD4WT group, using mAB OX68 (n = 6).

From: van Wilgenburg B, Moore MD, James WS, Cowley SA (2014)
The Productive Entry Pathway of HIV-1 in Macrophages Is Dependent on Endocytosis through Lipid Rafts Containing CD4.
PLoS ONE 9(1): e86071.
doi.10.1371/journal.pone.0086071
This image is from an open access article distributed under terms of a Creative Commo