Fig 2: Inhibition of Nrf2/HO-1 signaling pathway affected Th17/Treg cell ratio in AC mice with TWEAK knockdown. (A) The effect of Nrf2 inhibitor on Th17/Treg cell ratio in AC mice with TWEAK knockdown was assessed by flow cytometry. (B-C) WB and IHC assays were employed to evaluate the expression of FoxP3 and RORγt in conjunctival tissue of AC mice. (D) The levels of Th17 and Treg cytokines in mice spleen were detected by ELISA. *p < 0.05, **p < 0.01, ***p < 0.001 vs. AC + AAV-shNC; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. AC + AAV-shTWEAK