PTP sigma/PTPRS Antibody AF3430 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemPTP sigma/PTPRS Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $539.00Supplier Page View Company Product Page
Catalog NumberAF3430
NCBI Gene AliasesR-PTP-S, R-PTP-sigma, PTPSIGMA
ApplicationsFlow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunocytochemistry
ReactivityHu, Ms, Rt, Ck
ImmunogenMouse myeloma cell line NS0-derived recombinant human PTP sigma /PTPRS, Glu30-Gly1260, Accession # NP_570924
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
PurityAntigen Affinity-purified
ConcentrationLYOPH
ClonalityPolyclonal
NCBI Full Gene Nameprotein tyrosine phosphatase receptor type S
HostGoat
IsotypeIgG
Conjugate/TagUnconjugated
TargetPTP sigma/PTPRS
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects the endogenous cleaved extracellular domain of human PTP sigma /PTPRS in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human LAR, PTPRß, PTPR delta, PTPRµ, or PTPR kappa is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study the role of PTPRS in retinal function?

Yes, the PTP sigma/PTPRS antibody has been utilized in immunohistochemistry to investigate the role of PTPRS in retinal function, particularly in studies examining the signaling pathways involved in high-frequency light responses in mouse models ().

Is there evidence supporting the use of this antibody in cancer research?

The PTP sigma/PTPRS antibody has been referenced in studies focusing on colorectal cancer, where it was shown to regulate RAS pathway activity by modulating ERK phosphorylation and nuclear translocation, indicating its relevance in cancer research ().

What is the specificity of this antibody in detecting PTPRS?

The antibody specifically detects the endogenous cleaved extracellular domain of human PTPRS in Western blots, with less than 1% cross-reactivity observed with other related phosphatases, ensuring reliable results in experiments ().

How has this antibody been utilized in cellular signaling studies?

The PTP sigma/PTPRS antibody has been employed in various signaling studies, including those investigating the recruitment of myosin IIB to junctions in epithelial cells, highlighting its role in cell adhesion and signaling pathways ().

R&D Systems, a Bio-Techne Brand

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Phone: 1 (800) 343-7475
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Company Profile

Website: http://www.rndsystems.com

(1) PTPσ-mediated PI3P regulation modulates neurodegeneration in C9ORF72-ALS/FTDNeuronApril 16, 2025Zhe Zhang, Xiujuan Fu, Noelle Wright, Weiren Wang, Yingzhi Ye, Julie Asbury, Yini Li, Chengzhang Zhu, Rong Wu, Shaopeng Wang, et al.primary antibody in 5% BSA at 4 °C overnight. Primary antibodies are PTPσ (R & D Systems, Cat. No. AF3430, 1:1000), CC3 (CST, Cat. No. 96645, 1:1000), Rab5 (CST, Cat. No. 3547T,1:1000), EGFR (CST, Cat. No.

(2) ERG responses to high-frequency flickers require FAT3 signaling in mouse retinal bipolar cellsJ Gen PhysiolMarch 3, 2025Evelyn C. Avilés, Sean K. Wang, Sarina Patel, Sebastian Cordero, Shuxiang Shi, Lucas Lin, Vladimir J. Kefalov, Lisa V. Goodrich, Constance L. Cepko, Yunlu Xueand originally developed by Kirill Martemyanov Lab [Cao et al., 2009]), goat anti-PTPσ (1:200; Cat# AF3430; R&D Systems), rabbit anti-VGAT (1:300; Cat#131002; SynapticSystems), and mouse anti-VSX2 (Chx10, 1:100;

(3) Identification of the growth cone as a probe and driver of neuronal migration in the injured brainNature communicationsMarch 9, 2024Nakajima C, Sawada M, Umeda E, Takagi Y, Nakashima N, Kuboyama K, Kaneko N, Yamamoto S, Nakamura H, Shimada N, et al.

(4) High temporal frequency light response in mouse retina is mediated by ON and OFF bipolar cells and requires FAT3 signalingbioRxiv : the preprint server for biologyNovember 4, 2023Avilés EC, Wang SK, Patel S, Shi S, Lin L, Kefalov VJ, Goodrich LV, Cepko CL, Xue Y.USC and originally developed by Kirill Martemyanov Lab56), goat anti-PTPσ (1:200; R&D Systems, Cat# AF3430), rabbit anti-VGAT (1:300; SynapticSystems, Cat#131002), and mouse anti-VSX2 (Chx10, 1:100; Santa Cruz Biotechnology

(5) Autologous exosome facilitates load and target delivery of bioactive peptides to repair spinal cord injuryBioactive materialsJuly 1, 2023Ran N, Li W, Zhang R, Lin C, Zhang J, Wei Z, Li Z, Yuan Z, Wang M, Fan B, et al.

(6) Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizerNature communicationsOctober 14, 2020Apóstolo N, Smukowski SN, Vanderlinden J, Condomitti G, Rybakin V, Ten Bos J, Trobiani L, Portegies S, Vennekens KM, Gounko NV, et al.PA5-71105;RRID: AB_2689922 Rabbit anti-RPTPd,Novus,Cat#: NBP2-49153 Goat anti-RPTPs,R&D Systems,Cat#: AF3430;RRID: AB_2175157 Sheep anti-Teneurin-4,R&D Systems,Cat#: AF6320;RRID: AB_10920937 Mouse anti-PSA-NCAM1,Merck Millipore

(7) Roles of Collagen XXV and Its Putative Receptors PTPσ/δ in Intramuscular Motor Innervation and Congenital Cranial Dysinnervation DisorderCell reportsDecember 24, 2019Munezane H, Oizumi H, Wakabayashi T, Nishio S, Hirasawa T, Sato T, Harada A, Yoshida T, Eguchi T, Yamanashi Y, et al.

(8) PTPRS drives adaptive resistance to MEK/ERK inhibitors through SRCOncotargetNovember 26, 2019Davis TB, Yang M, Wang H, Lee C, Yeatman TJ, Pledger WJ.antibodies. Rabbit primary antibodies were used unless specified and were sourced as follows: PTPRS (goat AF3430 R&D Systems); alpha-Tubulin (mouse sc-8035 Santa Cruz). All other antibodies were obtained from Cell Signaling

(9) PTPRS Regulates Colorectal Cancer RAS Pathway Activity by Inactivating Erk and Preventing Its Nuclear TranslocationScientific reportsJune 18, 2018Davis TB, Yang M, Schell MJ, Wang H, Ma L, Pledger WJ, Yeatman TJ.rabbit unless specified and were sourced as follows: PTPRS (mouse Cat.No.ab55640 Abcam); PTPRS (goat AF3430 R&D Systems); alpha-Tubulin (mouse sc-8035 Santa Cruz). All other antibodies were obtained from Cell Signaling

(10) Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTPσ receptors promotes a beneficial inflammatory response following spinal cord injuryJournal of neuroinflammationMarch 20, 2018Dyck S, Kataria H, Alizadeh A, Santhosh KT, Lang B, Silver J, Karimi-Abdolrezaee S.

(11) Protein Tyrosine Phosphatase PTPRS Is an Inhibitory Receptor on Human and Murine Plasmacytoid Dendritic CellsImmunityAugust 18, 2015Bunin A, Sisirak V, Ghosh HS, Grajkowska LT, Hou ZE, Miron M, Yang C, Ceribelli M, Uetani N, Chaperot L, et al.

(12) An RPTPα/Src family kinase/Rap1 signaling module recruits myosin IIB to support contractile tension at apical E-cadherin junctionsMolecular biology of the cellApril 1, 2015Gomez GA, McLachlan RW, Wu SK, Caldwell BJ, Moussa E, Verma S, Bastiani M, Priya R, Parton RG, Gaus K, et al.MA); 8) mouse monoclonal (FG6) anti-PTP1B (Calbiochem, Billerica, MA); 9) rabbit pAb against RPTPσ (AF3430; R&D Systems); 10 and 11) rabbit pAb against c-Yes and mAb against c-Src were from Cell Signaling and

(13) Modulation of the proteoglycan receptor PTPσ promotes recovery after spinal cord injuryNatureFebruary 19, 2015Lang BT, Cregg JM, DePaul MA, Tran AP, Xu K, Dyck SM, Madalena KM, Brown BP, Weng YL, Li S, et al.

(14) The roles of neuronal and glial precursors in overcoming chondroitin sulfate proteoglycan inhibitionExperimental neurologyJune 1, 2012Ketschek AR, Haas C, Gallo G, Fischer I.staining, cultures were incubated with anti-beta III tubulin (TUJ1) and either anti-hPTPRσ (goat IgG 1:100; AF3430, R&D Systems) or anti-LAR (H-70) (rabbit IgG 1:200; sc-25434, Santa Cruz). All cultures were stained

(15) Synoviocyte-targeted therapy synergizes with TNF inhibition in arthritis reversalScience advancesJanuary 1, 0001"Svensson MND, Zoccheddu M, Yang S, Nygaard G, Secchi C, Doody KM, Slowikowski K, Mizoguchi F, Humby F, Hands R, et al."

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