Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using 039739 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 039739 for immunohistochemistry.

Confocal immunofluorescent analysis with NCI-H460 cells using 039739 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Flow Cytometric analysis of NCI-H460 cells using 039739 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.