Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb 9496L from Cell Signaling Technology

Product Specs
ItemCleaved Caspase-8 (Asp374) (18C8) Rabbit mAb
CompanyCell Signaling Technology
Price $947.00 Inquire
Catalog Number9496L
Quantity300 µl
ApplicationsW
ReactivityH
TargetCasp8 (Asp374) cleaved
HostRabbit
NCBI Gene AliasesALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5
ClonalityMonoclonal
NCBI Full Gene Namecaspase 8
Research AreaApoptosis
IsotypeIgG
Clone18C8
Molecular Weight18, 41, 43

Questions and Answers

Can this antibody be used to study apoptosis in human cells?

Yes, the Cleaved Caspase-8 (Asp391) Rabbit mAb has been shown to be effective in detecting cleaved caspase-8, a key marker of apoptosis, in various studies. For instance, it was utilized in research examining the apoptotic effects of different treatments on cancer cells, highlighting its relevance in apoptosis studies (, ).

What specific applications has this antibody been cited in?

The Cleaved Caspase-8 antibody has been cited in multiple applications, primarily in Western Blot analysis. It has been used to assess apoptosis in various experimental contexts, including studies on lung cancer and trophoblast apoptosis (, ).

Is this antibody suitable for use in different types of cancer research?

Yes, the Cleaved Caspase-8 antibody has been referenced in studies involving various cancer types, including lung cancer and adult T-cell leukemia/lymphoma. Its ability to detect cleaved caspase-8 makes it a valuable tool for investigating apoptotic pathways in these malignancies (, ).

Are there any known limitations or specific conditions for using this antibody?

While the Cleaved Caspase-8 antibody is widely applicable, it is important to note that its effectiveness may vary depending on the specific cell line or experimental conditions. For example, in some studies, the antibody detected a band of unknown origin at 25 kDa in certain cell lines, which may require further investigation ().

Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Cell autonomous inflammation in VEXAS is mediated by cGAS-STINGbioRxivMay 29, 2026Samuel J. Magaziner, Jason C. Collins, Brecca Miller, Patrick Zheng, Amy K. Wang, Jerome Hadjadj, Juan Carlos Baladrán, Maria Sirenko, Maya English, James Bertlin, et al.Caspase 3 (Cell Signaling, 9661S), Caspase 8 (Cell Signaling, 4790S), Cleaved Caspase 8 (Cell Signaling, 9496S), RIPK1 (Cell Signaling, 3493S), phospho-RIPK1 (Cell Signaling, 65746S), MLKL (Cell Signaling, 14993S),

(2) Proteasomal inhibition compromises microvascular integrity via distinct effects on non-immune endothelial cells and immune cellsFrontiers in ImmunologyMay 19, 2026Prajakta Sawant, Hannah Wolf, Aleena Mathew, Johanna Bensalel, Julio Gallego-Delgado, Pratyusha MandalCl-CASP3 (Cat# D175 9661, RRID: AB_2341188), Cl-CASP9 (Cat# 20750, RRID: AB_2798848), Cl-CASP8 (Cat# 9496, RRID: AB_561381), LC3B (Cat# 83506, RRID: AB_2800018), PERK, IRE1a, CHOP, BiP (ER Stress Kit Cat# 9956),

(3) GO-Y078 Triggers Program Cell Death in Human Cervical Cancer Cells Via MAPK Activation-Dependent Apoptotic Caspases SignalingIntegrative Cancer TherapiesApril 30, 2026Chung-Yuan Lee, Po-Hui Wang, Yi-Hsien Hsieh, Yi-Hsuan Hsiao, Chih-Hsin Tang, Tzu-Yu Huang, Shun-Fa Yang, Pei-Ni Chen(ADP-ribose) polymerase (PARP; CST, Cat #9542, RRID: AB_2160739), anti-cleaved caspase-8 (CST, Cat #9496, RRID: AB_561381), anti-cleaved caspase-9 (CST, Cat #9505, RRID: AB_2290727), anti-cleaved caspase-3

(4) βIII-tubulin can act as a brake on extrinsic apoptosis in pancreatic cancerCell Death & DiseaseApril 24, 2026George Sharbeen, John Kokkinos, Grace Schulstad, Elvis Pandzic, Janet Youkhana, Zerong Ma, Rosa Mistica C. Ignacio, Aparna S. Raina, Shannon Chiang, Cyrille Boyer, et al.described [12, 23, 32]; (ii) cleaved caspase 8 primary antibody (1:100; Cell Signalling Technology, cat. #9496) and goat anti-rabbit biotinylated secondary (1:200; Vector Laboratories, cat. BA-1000). Quantification

(5) Cryptocaryone Exhibits ROS/MAPK-Dependent Antiproliferative and Apoptosis-Inducing Effects on Triple-Negative Breast Cancer Cells and Proof-of-Concept Breast Cancer Mouse ModelDrug Dev ResApril 19, 2026Ya-Ting Chuang, Wangta Liu, Tsu-Ming Chien, Ammad Ahmad Farooqi, Hsun-Shuo Chang, Jun-Ping Shiau, Hsueh-Wei ChangAntibodies (1:1000) were derived from an apoptosis sampler antibody kit [#9915] and a cleaved caspase 8 [#9496] (Cell Signaling Technology), accompanied by the internal control antibody GAPDH (1:10000) [#GT239]

(6) Antigenic cancer persister cells survive direct T cell attackbioRxivApril 12, 2026Michael X. Wang, Brandon E. Mauch, August F. Williams, Tania Barazande-Pour, Filipe Araujo Araujo Hoffmann, Sophie H. Harris, Cooper P. Lathrop, Claire E. Turkal, Bryan S. Yung, Michelle H. Paw, et al.(CST #9176, 1:1000), PD-L1 (CST #13684, 1:1000), IDO-1 (CST #86630, 1:1000), Cleaved Caspase 8 (CST #9496, 1:1000), Cleaved Caspase 7 (CST #9491, 1:1000), Cleaved Caspase 3 (CST #9664, 1:1000), Phospho-Histone

(7) Lipid droplet-induced T cell death sustains autoimmune tissue inflammationCell MetabolismApril 7, 2026Jitendra Kumar, Yoshinori Takashima, Jose Morales, Brandon Wong, Lina Werner, Isabel N. Goronzy, Selene Rubino, Robert T. Trousdale, Gerald J. Berry, Jörg J. Goronzy, et al.,Cell Signaling Technology,#8438; P55210 Caspase-8 Cleaved (Asp374) (18C8) Rabbit mAb,Cell Signaling Technology,#9496; Q14790 CD44 Antibody,Cell Signaling Technology,#3578: P16070 Cleaved Gasdermin D (Asp275) (E7H9G) Rabbit mAb

(8) UPR/ATF4/Noxa pathway overactivation through SERCA2 inhibition or ONC201 treatment combined with ABT-737 triggers apoptosis in chemoresistant ovarian cancer cells and patient-derived tumor organoidsCell Death & DiseaseMarch 27, 2026Sahra Messaoudi, Romane Florent, Louis-Bastien Weiswald, Guillaume Desmartin, Lucie Lecouflet, Steven Lohard, Léa Grunchec, Edwige Abeilard, Marilyne Guillamin, Jordane Divoux, et al.(#2774), anti-BCL-xL (#2764), anti-BIM (#2819), anti-BiP (#3177), anti-CASPASE 3 (#9662), anti-CASPASE 8 (#9496), anti-CHOP (#2895), anti-DR5 (#8074), anti-MCL-1 (#5453), anti-PARP (#9542), anti-PUMA (#12450) (Cell Signaling Technology

(9) Macrophage metabolic exhaustion and PANoptotic cell death drive chronic tissue inflammation in rheumatoid arthritisImmunityMarch 6, 2026Tao Huang, Yoshinori Takashima, Jitendra Kumar, Jose Morales, Kevin Le, Zhensheng Hu, Selene Rubino, Robert T. Trousdale, Gerald J. Berry, Jorg J. Goronzy, et al.(KP1) (ThermoFisher Scientific, 14–0688-82), cleaved caspase-7 (CST, 9491S), cleaved caspase-8 (CST, 9496S), goat anti-rat IgG (H+L) Cyanine3 (Invitrogen, A10522), donkey anti-sheep IgG (H+L) Alexa Fluor 647

(10) Notch-1 in macrophages promoted the ischemia-reperfusion via modulating EZH2/HSF1/BRD4/SIRPα/SHP2 induced ROS and apoptosis in cardiomyocyteScientific ReportsFebruary 25, 2026Chengbi Tong, JiaWei Zhang, YaJing Zuo, XiaoTong Yu, ShaSha Zang, Sha Liucleaved-caspase3(CST, #9664, 1:1000), Caspase-8(CST, #4790, 1:1000), GAPDH(CST, #2118, 1:2000) cleaved-caspase8(CST, #9496, 1:1000), and PCNA(CST, #13110, 1:1000) at 4 °C overnight. After adding secondary antibodies tagged

(11) Caspase-activation powers anti-Desmoglein 3-induced acantholysis in human epidermisCell Death DiscoveryFebruary 19, 2026Morna F. Schmidt, Maria A. Feoktistova, Diana Panayotova-Dimitrova, Eva Miriam Buhl, Peter Boor, Tim Ruhl, Jens Waschke, Ritva Tikkanen, Martin Röcken, Jens M. Baron, et al.Mouse IgG1 Alexa Fluor® 488-conjugated Antibody (IC002G, R&D, Minneapolis, USA), Cleaved caspase-8 (#9496, Cell Signaling, Danvers, USA), anti-Desmoglein 3 antibody (ab183743, Abcam, Cambridge, United Kingdom).

(12) A Novel Pyrazole Pyrimidine Derivative MBP346 Induces Cell Death via ROS-Mediated Mitochondrial Damage in Human Head and Neck Squamous Cell CarcinomaMoleculesFebruary 17, 2026Chunsheng Hu, Pengcheng Xu, Juan Hu, Bo Fang, Jiangping Meng, Yan Tang(9532, CST, Danvers, MA, USA), Cleaved-Caspase 3 (9664, CST, Danvers, MA, USA), Cleaved-Caspase 8 (9496, CST, Danvers, MA, USA), MCL1 (220062, Zen BIO, Chengdu, China), Bcl2 (12789-1-AP, Proteintech, Wuhan,

(13) Ebastine targets HER2/HER3 signaling and cancer stem cell traits to overcome trastuzumab resistance in HER2‑positive breast cancerInt J Mol MedFebruary 2, 2026Eunsun Jung, Ji Young Kim, Dongmi Ko, Juyeon Seo, Sang Yoon Lee, Daeun Lee, Subeen Kim, Minsu Park, Seongjae Kim, Soeun Park, et al.(Cell Signaling Technology, Inc.; cat. no. 8438) and -8 (Cell Signaling Technology, Inc.; cat. no. 9496), Bax (Cell Signaling Technology, Inc.; cat. no. 2772) and vimentin (Cell Signaling Technology, Inc.;

(14) EGCG promotes apoptosis in BT-549 triple-negative breast cancer cells by targeting STAT3Translational Cancer ResearchJanuary 31, 2026Ke Zhang, Xinghua Tan, Xufeng Zhou, Lushan Yu, Zhihua Tangcaspase-3 (CST, 9662), cleaved caspase-3 (CST,9661), caspase-8 (CST, 4790), cleaved caspase-8 (CST, 9496), ERK (CST, 4695), p-ERK (CST, 5726), JAK (CST, 3332), p-JAK (CST,3331), GAPDH (CST, 2118). Flow cytometry

(15) Epithelial Gab1 Restricts Sepsis‐Induced Intestinal Injury by Orchestrating TNF/NF‐κB AxisMediators of InflammationJanuary 31, 2026Wei Jin, Yanchuang Wu, Xiaoqing Cheng, Yu Pan, Lifeng He, Yun Xu, Jiaqi Xu, Xue Zhang, Hengrui Liu14064‐1‐AP, 1:1000) cl‐Caspase3 (CST, 9664, 1:1000), Caspase3 (CST, 9662, 1:1000), cl‐Caspase8 (CST, 9496, 1:1000), Caspase8 (CST, 4790, 1:1000), Bcl‐2 (Proteintech, 12789‐1‐AP, 1:1000), Bax (Proteintech, 50599‐2‐Ig,

(16) Homoisoflavanone Delays Colorectal Cancer Progression via DNA Damage‐Induced Mitochondrial Apoptosis and Parthanatos‐Like Cell DeathAdvanced ScienceJanuary 28, 2026Hongjie Fan, Huzi Zhao, Pei Zhang, Pengfei Yu, Yunfei Ji, Gang Chen, Hongli Jin, Yanfang Liu, Jin Liu, Zhe‐Sheng Chen, et al.antibodies against the following proteins: cleaved Caspase 3 (25128‐1‐AP, Proteintech), cleaved Caspase 8 (9496, CST), PARP (9542, CST), cleaved PARP (9541, CST), P21 (2947, CST), P53 (2527, CST), Cyclin A2 (91500,

(17) Pharmacologic Modulation of the PAR-2–ERK Axis by Statins Converts Inflammatory Survival Signalling into Apoptosis in Colorectal Cancer CellsInternational Journal of Molecular SciencesJanuary 16, 2026Layla Amiri, Rajashree Patnaik, Riah Lee Varghese, Bintul Huda, Yajnavalka Banerjeecleaved caspase-3 (25128-1-AP, Proteintech), pro-caspase-8 (13423-1-AP, Proteintech), cleaved caspase-8 (9496T, Cell Signaling), BCL-2 (3498T, Cell Signaling), BAX (50599-2-Ig, Proteintech), and GAPDH (2118T, Cell Signaling

(18) A Novel Autophagy Inhibitor p -Hydroxylcinnamaldehyde Suppresses Esophageal Squamous Cell Carcinoma by Targeting LDHA Phosphorylation-Mediated Metabolic ReprogrammingResearch (Wash D C)January 12, 2026Sisi Wei, Jingjing Wang, Zhe Zhang, Yuhui Fu, Leyang Zhao, Yanna Bi, Xiaoya Li, Suli Dai, Cong Zhang, Wenjiao Zhu, et al.and antibodies Antibodies against cleaved caspase-3 (#9664), caspase-3 (#9662), cleaved caspase-8 (#9496), caspase-8 (#4790), cleaved caspase-9 (#9505), caspase-9 (#9508), p-AMPK (#2535), and p-ULK1 (#14202)

(19) Targeting FOXM1 regulates metabolic signatures through ROS-dependent JNK/Bmi1/Skp2 axis in human cutaneous T-cell lymphomaCell Death & DiseaseJanuary 7, 2026Abdul Q. Khan, Maha Agha, Fareed Ahmad, Rasheeda Anver, Majid Alam, Joerg Buddenkotte, Shahab Uddin, Martin Steinhoff9252), FOXM1 (Cat# 20459), Caspase-3 (Cat# 9662), Cleaved caspase-3 (Cat# 9661), Cleaved caspase-8 (Cat# 9496), Skp2 (Cat# 4358), Bmi1(Cat# 6964), p21 (Cat# 64016), PARP (Cat# 9542), Cleaved PARP (Cat# 9544), LC3A/B

(20) Betulinic Acid Suppresses UBE2T Expression via MAPK/ERK Inhibition to Block FANCI and FANCD2 Monoubiquitination in GlioblastomaJ Cell Mol MedJanuary 4, 2026Yifeng Bao, Maode WangNo. 8033), UAF1 (Abcam, Cat. No. ab122473), Pro‐Caspase 8 (Asp374), p43/p41 and p18 (CST, Cat. No. 9496), YY1 (CST, Cat. No. 46395), Lamin B1 (CST, Cat. No. 13435), ERβ (CST, Cat. No. 89954), pERK1/2 (Thr202/Tyr204)

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6/7/2022Great Cleaved Caspase-8 for Western Blot Daniel HamiltonEpithelial cells were seeded. After 24 hours I used starvation medium (0% FBS) for 4 hours and I treated with eiF4E siRNA for 24 hours with 2% FBS medium. The western blot membrane was incubated ... Read Review

Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb

Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb from Cell Signaling Technology

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