STIM1 (D88E10) Rabbit mAb 5668S from Cell Signaling Technology

Product Specs
ItemSTIM1 (D88E10) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number5668S
Quantity100 µl
ApplicationsW, IP, IF-IC
ReactivityH, M, R, Mk, B
TargetSTIM1
IsotypeIgG
HostRabbit
NCBI Gene AliasesD11S4896E, GOK, IMD10, STRMK, TAM, TAM1
NCBI Full Gene Namestromal interaction molecule 1
CloneD88E10
Molecular Weight85
ClonalityMonoclonal
Research AreaCa, cAMP and Lipid Signaling

Questions and Answers

What species can I use this STIM1 antibody on for my experiments?

The STIM1 (D88E10) Rabbit mAb recognizes endogenous levels of total STIM1 protein and is reactive with several species, including human, mouse, rat, bovine, and non-human primates.

Has this antibody been used in any specific applications in the literature?

Yes, the STIM1 antibody has been shown to work in various applications, including Western blotting, immunocytochemistry, immunofluorescence, and immunoprecipitation, as documented in multiple studies.

Can this antibody help in studying calcium signaling in neuronal development?

Indeed, research has demonstrated that STIM1 plays a crucial role in store-operated calcium entry (SOCE) in human neural precursor cells, influencing their self-renewal and differentiation, as shown in studies focusing on calcium signaling in neuronal development.

Is there any evidence of STIM1's role in disease mechanisms?

Yes, STIM1 has been implicated in various disease mechanisms, including its interaction with the STING pathway, which is essential for the type I interferon response. Deficiency in STIM1 has been linked to enhanced expression of type I interferons, which can contribute to autoimmune conditions.

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Website: Technical Support

(1) Lysine attenuates acute lung injury by restoring α-tubulin acetylation and ciliary activityCell Death DiscoveryMarch 16, 2026Wenyu Yang, Xiaoxiao Meng, Yong Zhu, Xinrun Ma, Zhuoran Cao, Mengmeng Wang, Wentao Dai, Yiming Tao, Xiangdong Jian, Rui Tian, et al.Primary antibodies were incubated at 4 °C overnight, including Anti-STIM1 (Cell Signaling Technology, 5668, 1:3000), Anti-ORAI1 (Santa Cruz, sc-377281, 1:500), Anti-TRPC1 (Proteintech, 19482-1-AP, 1:2000), Anti-E-Cadherin

(2) Deletion of STIM1 in Treg cells protects against lung fibrosis and associated cardiovascular complications in a pre-clinical mouse modelRedox BiolMarch 11, 2026Nagaraja Nagre, Balaji Srinivas, Nicholas Richards, Ryan Washington, Gregory Nicholson, Yan Sanders, Beata Kosmider, Khalid MatrouguiWestern Blot Substrate (ThermoFisher Scientific). The following primary antibodies were used: anti-STIM1 (5668, 1:500), anti-FoxP3 (12632, 1:500), from Cell Signaling Technology Inc., anti-p-eNOS (612393, 1:500),

(3) The Ca2+ sensor STIM1 promotes neuronal ferroptosis by regulating iron homeostasis to exacerbate brain injury after intracerebral hemorrhageCell Reports MedicineFebruary 17, 2026Hongchen Zhang, Lijun Ma, Zheming Yue, Chuanhao Lu, li Wang, Min Zhang, Jia Yong, Yuan Feng, Shiquan Wang, Hongjie Wang, et al.normalized to β-actin densities for the same samples.The following primary antibodies were used: STIM1 (5668, Cell Signaling Technology, USA), β-actin (Cell Signaling Technology, 3700, USA), GPX4 (A11243, ABclonal,

(4) STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondriaThe EMBO JournalFebruary 11, 2026Yolanda Orantos-Aguilera, Irene Sanchez-Lopez, Carlos Pascual-Caro, Patricia Gómez-Suaga, Estela Area-Gomez, Eulalia Pozo-Guisado, Jorge Montesinos, Francisco Javier Martin-Romeroat 4 °C. Clarified lysates were incubated with 5 μl anti-STIM1 antibody (Cell Signaling Technology, #5668) in binding buffer (10 mM NaH2PO4 pH 7.0, 140 mM NaCl, 0.05% Triton X-100) overnight at 4 °C with gentle

(5) Dual roles of TRPV2 in the innate immune response to cytosolic DNA: Arresting dormant and boosting activated STINGCell ReportsJanuary 27, 2026Chen Cheng, Hsiang-Ting Lu, Shan Li, Zhongsheng You,Cell Signaling Technology,Cat# 67591; RRID: AB_2904212 Rabbit anti-STIM1,Cell Signaling Technology,Cat# 5668; RRID: AB_10828699 Rabbit anti-TREX1,Cell Signaling Technology,Cat# #15107; RRID: AB_2798710 Rabbit

(6) A novel miniTurbo knock-in mouse reveals a protein interaction network of USP46 in the brainExperimental AnimalsJanuary 1, 2026Kazuya Murata, Noa Haneishi, Reiko Nakagawa, Yoko Daitoku, Seiya Mizuno(26778-1-AP; 1:2,000, Proteintech), anti-PPP1R9B (14136; 1:1,000, Cell Signaling Technology), anti-STIM1 (5668; 1:1,000, Cell Signaling Technology), and anti-PLPP3 (27391-1-AP; 1:1,000, Proteintech). Streptavidin

(7) Altered Mitochondrial Bioenergetics and Calcium Kinetics in Young-Onset PLA2G6 Parkinson's Disease iPSCsJ NeurochemApril 6, 2025Thasneem Musthafa, Syed Kavish Nizami, Ankita Mishra, Gaiti Hasan, Renjitha Gopurappillysystem at 15 V for 15 min. The blots were incubated with STIM1antobody (Cell Signaling Technology, Cat# 5668S, RRID:AB_10828699) and β‐Tubulin (1:3000, DSHB E7, Cat# AB_2315513, RRID:AB_528499) overnight. Secondary

(8) Ca2+ tunneling architecture and function are important for secretionThe Journal of cell biologyJanuary 6, 2025Courjaret RJ, Wagner LE, Ammouri RR, Yule DI, Machaca K.following primary antibodies were used at a 1:500 dilution: SERCA2 (NB300-581; Novus Biologicals), STIM1 (5668S; Cell Signaling and MAI-19451; Thermo Fisher Scientific), KRAP (14157-1-AP; Thermo Fisher Scientific),

(9) Redox Enzymes P4HB and PDIA3 Interact with STIM1 to Fine-Tune Its Calcium Sensitivity and ActivationInternational Journal of Molecular SciencesJuly 10, 2024Yangchun Du, Feifan Wang, Panpan Liu, Sisi Zheng, Jia Li, Rui Huang, Wanjie Li, Xiaoyan Zhang, Youjun Wanganti-HRP-labeled Streptavidin (N100, Thermo Fisher scientific, Waltham, MA, USA), anti-STIM1 antibody (5668S; CST, Danvers, MA, USA) (1:1000 dilution), anti-PDIA3 antibody (159678-1-AP; Proteintech, Rosemont,

(10) Rapamycin Controls Lymphoproliferation and Reverses T-Cell Responses in a Patient with a Novel STIM1 Loss-of-Function DeletionJournal of Clinical ImmunologyMay 1, 2024Ibrahim Serhat Karakus, Mehmet Cihangir Catak, Alexandra Frohne, Feyza Bayram Catak, Melek Yorgun Altunbas, Royala Babayeva, Sevgi Kostel Bal, Sevgi Bilgic Eltan, Ezgi Yalcin Gungoren, Fehim Esen, et al.primary antibody overnight at 4℃. The following primary (STIM1—1:1000, Cell Signaling Technology, #5668; β-actin—1:5000, Sigma Aldrich, #A1978; HSP90α/β—1:5000, Santa Cruz Biotechnology, #sc-13119) and secondary

(11) STIM1 translocation to the nucleus protects cells from DNA damageNucleic acids researchMarch 21, 2024Sanchez-Lopez I, Orantos-Aguilera Y, Pozo-Guisado E, Alvarez-Barrientos A, Lilla S, Zanivan S, Lachaud C, Martin-Romero FJ.at 4°C. Clarified lysates were incubated with 5 μl anti-STIM1 antibody (Cell Signaling Technology, #5668) in binding buffer (10 mM NaPO4 pH 7.0, 140 mM NaCl, 0.05% Triton X-100) overnight at 4°C with gentle

(12) Stromal interaction molecule 1/microtubule‐associated protein 1A/1B‐light chain 3B complex induces metastasis of hepatocellular carcinoma by promoting autophagyMedComm (2020)February 9, 2024Jingchun Wang, Qichao Xie, Lei Wu, Yu Zhou, Yanquan Xu, Yu Chen, Jiangang Zhang, Ran Ren, Shiming Yang, Yongsheng Li, et al.coimmunoprecipitation LC3B antibody (3868S), N‐cadherin antibody (13116S), E‐cadherin antibody (3195S), STIM1 antibody (5668S), SQSTM1/p62 antibody (23214S), ZEB antibody (83243SF), MMP‐2 antibody (40994S), Vimentin antibody (5741S),

(13) Shank3 ameliorates neuronal injury after cerebral ischemia/reperfusion via inhibiting oxidative stress and inflammationRedox biologyFebruary 1, 2024Zhang H, Feng Y, Si Y, Lu C, Wang J, Wang S, Li L, Xie W, Yue Z, Yong J, et al.anti-shank3 (Neuromab, Cat# 75344, 1:200), rabbit anti-STIM1 (1:100, Cell Signaling Technology, Cat# 5668), rabbit anti-NeuN (1:100, Abcam, Cat# ab177487). After rinsing three times with PBS, sections or cells

(14) Diacylglycerol kinase-ε is S-palmitoylated on cysteine in the cytoplasmic end of its N-terminal transmembrane fragmentJournal of lipid researchJanuary 1, 2024Traczyk G, Hromada-Judycka A, Świątkowska A, Wiśniewska J, Ciesielska A, Kwiatkowska K.(Cell Signaling, catalog no.: 2276) for mDGKε-Myc staining and anti-STIM1 rabbit IgG (Cell Signaling, catalog no.: 5668) or anti-golgin-97 rabbit IgG (Cell Signaling, catalog no.: 13192) or anti-GM130 rabbit IgG (Cell Signaling,

(15) Spatacsin regulates directionality of lysosome trafficking by promoting the degradation of its partner AP5Z1PLOS BiologyOctober 23, 2023Alexandre Pierga, Raphaël Matusiak, Margaux Cauhapé, Julien Branchu, Lydia Danglot, Maxime Boutry, Frédéric Darios(Cat#6556, Abcam), mouse anti-Myc (clone 9E10, Development Studies Hybridoma Bank), rabbit anti-Stim1 (Cat#5668, Cell Signaling Technology), rabbit anti-cathepsin D (Cat#Ab75852, Abcam), rabbit anti-spatacsin (Cat#16555-1-AP,

(16) Regulation of store-operated Ca(2+) entry by IP(3) receptors independent of their ability to release Ca(2)eLifeJuly 19, 2023Chakraborty P, Deb BK, Arige V, Musthafa T, Malik S, Yule DI, Taylor CW, Hasan G.(1:5000, BD Biosciences, Cat# 612656, RRID: AB_2289199); STIM1 (1:1000, Cell Signaling Technology, Cat# 5668 S, RRID: AB_10828699); Orai1 (1:500, ProSci, Cat# PM-5205, RRID: AB_10941192); IP3R2 (1:1000, custom

(17) Impaired intracellular calcium homeostasis enhances protein O-GlcNAcylation and promotes vascular calcification and stiffness in diabetesRedox BiologyJuly 1, 2023Weiping Zhang, Yong Sun, Youfeng Yang, Yabing ChenWestern blot analysis Western blot analyses were performed with the use of specific antibodies for STIM1 (Cell signaling, 5668S), α-SMA (Sigma, A5228), Col1a (Sigma, SAB2100463), Runx2 (MBL, D130-3), O-GlcNAcylation (RL-2; Abcam),

(18) Vitamin K-dependent carboxylation regulates Ca(2+) flux and adaptation to metabolic stress in β cellsCell reportsMay 30, 2023Lacombe J, Guo K, Bonneau J, Faubert D, Gioanni F, Vivoli A, Muir SM, Hezzaz S, Poitout V, Ferron M.per condition, from 3 independent experiments). Islet clusters stained for STIM1 (rabbit anti-STIM1; 5668; Cell Signaling) contained on average 0–57 puncta at steady state (KRBH buffer with 5mM glucose). The

(19) Rescue of astrocyte activity by the calcium sensor STIM1 restores long-term synaptic plasticity in female mice modelling Alzheimer's diseaseNature communicationsMarch 22, 2023Lia A, Sansevero G, Chiavegato A, Sbrissa M, Pendin D, Mariotti L, Pozzan T, Berardi N, Carmignoto G, Fasolato C, et al.n. B286227); anti-STIM1 (RRID: AB10828699, 1:400 monoclonal in rabbit, clone D88E10, Cell Signaling, 5668); anti-NeuN (RRID: AB_2298772, 1:100 monoclonal in mouse, A60 clone, Merck-Millipore MAB377, Lot n.

(20) Huntingtin regulates calcium fluxes in skeletal muscleThe Journal of general physiologyJanuary 2, 2023Chivet M, McCluskey M, Nicot AS, Brocard J, Beaufils M, Giovannini D, Giannesini B, Poreau B, Brocard J, Humbert S, et al.immunoprecipitation), antibodies against STIM1 from Merck Millipore (ref AB9870) and Ozyme (clone D88E10; ref 5668S; Cell Signaling Technology), antibodies against JPH1 from Sigma-Aldrich (ref. WH0056704M4) and Invitrogen

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7/13/2022Chemoattractant Effect on STIM1 Expression Somasundaram RaghavanSTIM1 is a ubiquitously expressed highly conserved protein. It regulates the Ca2+ sensor of endoplasmic reticulum that shifts from the ER Ca2+ store to the plasma membrane, which inturn activates the... Read Review

STIM1 (D88E10) Rabbit mAb

STIM1 (D88E10) Rabbit mAb from Cell Signaling Technology

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STIM1 (D88E10) Rabbit mAb from Cell Signaling Technology

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