Immunohistochemistry (Paraffin) analysis of beta Amyloid in paraffin embedded human hippocampus affected by Alzheimers disease using beta Amyloid Chimeric Monoclonal Antibody (Product # MA5-48043). Antigen retrieval was achieved by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Tissue was stained with beta Amyloid Chimeric Monoclonal Antibody at a concentration of 4 µg/mL for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3-diaminobenzidine), and counter-staining with hematoxylin. Recommended concentration, 2-4 µg/mL.