Immunocytochemistry/Immunofluorescence analysis of CD299 in HeLa cells using CD299 (DC-SIGN/L) Chimeric Monoclonal Antibody (Product # MA5-47780). Paraformaldehyde-fixed cells were stained with CD299 (DC-SIGN/L) Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor488 secondary antibody (1 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom CD299 (DC-SIGN/L) Chimeric Monoclonal Antibody, DAPI, merged channels and an isotype control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor488 secondary antibody.