CR1/CD35 (E8B1Z) Rabbit mAb 96615S from Cell Signaling Technology

Product Specs
ItemCR1/CD35 (E8B1Z) Rabbit mAb
CompanyCell Signaling Technology
Price $402.00 Inquire
Catalog Number96615S
Quantity100 µl
ApplicationsW, IHC-P
ReactivityH
HostRabbit
TargetCR1
ClonalityMonoclonal
NCBI Full Gene Namecomplement C3b/C4b receptor 1 (Knops blood group)
NCBI Gene AliasesC3BR, C4BR, CD35, KN

Questions and Answers

Has this antibody been used in any studies related to apoptosis?

Yes, the CR1/CD35 Rabbit monoclonal antibody has been utilized in studies investigating apoptosis. For instance, it was employed to detect cleaved caspase-3 in various experimental setups, indicating its role in apoptosis pathways as demonstrated in multiple publications, including those focusing on renal toxicity and cancer research.

Can this antibody be used for immunohistochemistry, and if so, how has it been applied in research?

The CR1/CD35 antibody has been shown to work in immunohistochemistry applications. It has been used to assess apoptosis in tissue samples, such as in studies examining the effects of diabetes on fracture healing, where it was applied to evaluate cleaved caspase-3 levels in chondrocytes.

What species does this antibody react with, and how has this been validated in research?

This antibody is reactive with human tissues, specifically Homo sapiens. Its specificity for human proteins has been confirmed in various studies, including those that utilized the antibody to analyze human prostate cancer cells and other human-derived samples.

Are there any specific experimental conditions or protocols recommended for using this antibody in Western blotting?

While specific experimental protocols are not detailed in the provided information, the CR1/CD35 antibody has been cited in studies that employed Western blotting to analyze protein expression levels, including cleaved caspase-3. Researchers typically follow standard protocols for protein extraction, gel electrophoresis, and transfer to membranes, followed by incubation with the antibody for detection.

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) ENN A1 and B1 In Vitro Toxicological Effects on 2D and 3D Organ-on-Chip HepaRG Liver CellsToxins (Basel)February 20, 2026France Coulet, Monika Coton, Elena Refet-Mollof, Emmanuel Coton, Thomas Gervais, Nolwenn HymeryWithby, ON, Canada), or rabbit anti-CC3 antibodies (targeting the activated form of caspase-3) (1:600) (96615, ThermoFisher Scientific, Waltham, MA, USA) overnight at 4 °C. After one wash with PBS, the wells or

(2) Remodelling of supernumerary leaflet primordia leads to bicuspid aortic valve (BAV) caused by loss of primary ciliaCardiovascular ResearchSeptember 29, 2025Ahlam Alqahtani, Lorraine Eley, Jake Newton, Kimberley Macdonald, Chloe Connolly, Cindy Rodrigues-Cleto, Kristyna Neffeova, Leonor Lopez, Javier Arias, Christopher J Derrick, et al.(abcam; ab8296), Notch3 (abcam; ab23426), Lef1 (abcam; ab2230), Axin2 (abcam; ab32197), Caspase3 (abcam, 96615), Collagen I (abcam; ab21286), Versican (abcam; ab1032), Periostin (abcam; ab14041), and BrdU (abcam;

(3) Pregnancy enhances antiviral immunity independent of type I IFN but dependent on IL-17–producing γδ + T cells in the nasal mucosaScience AdvancesSeptember 27, 2024Julia Chronopoulos, Erwan Pernet, Kim A. Tran, Toby K. McGovern, Arina Morozan, Sadie Wang, Oscar Tsai, Kosuke Makita, Maziar Divangahi, James G. Martinwere deparaffinized, rehydrated, and incubated for 24 min at 37°C with anti–cleaved caspase-3 (1:300; #96615, Cell Signaling) and then incubated with secondary antibody OmniMap anti-rabbit horseradish peroxidase

(4) FOXO 1 deletion in chondrocytes rescues diabetes-impaired fracture healing by restoring angiogenesis and reducing apoptosisFront Endocrinol (Lausanne)August 2, 2023Mohammed A. Alharbi, Dana T. GravesCD-31 specific antibody (Abcam, ab28364), anti-cleaved caspase 3 antibody (Cell Signaling Technology 96615) or the appropriate isotype-matched negative control IgG (Vector, I-1000, Burlingame, CA). The primary

(5) Evaluation of expanded peripheral blood derived CD34+ cells for the treatment of moderate knee osteoarthritisFrontiers in Bioengineering and BiotechnologyMay 23, 2023Christine Vignon, Morgane Hilpert, Karine Toupet, Aurélien Goubaud, Danièle Noël, Matthieu de Kalbermatten, Philippe Hénon, Christian Jorgensen, Andrea Barbero, Ibon Garitaonandiafor the detection of CD45+ (Abcam, cat# Ab133469) human cells and caspase 3+ (Cell signaling, cat# 96615, clone ASP175) apoptotic cells on serial sections. Slides were scanned using the Nanozoomer 2.0 Hamamatsu

(6) Zinc finger protein‑like 1 is a novel neuroendocrine biomarker for prostate cancerInternational journal of oncologyMarch 1, 2023Masud N, Aldahish A, Iczkowski KA, Kale A, Shah GV.and CD63 (cat. no. SAB4700215); both from Sigma-Aldrich; Merck KGaA]; [cleaved caspase 3 (cat. no. 96615), Phospho (p)-AktThr308 (cat. no. 92755) Phospho-AktSer473 (cat. no. 92715) and Akt (cat. no. 9272);

(7) Human immature testicular tissue organ culture: a step towards fertility preservation and restorationFrontiers in endocrinologyJanuary 1, 2023Younis N, Caldeira-Brant AL, Chu T, Abdalla S, Orwig KE.anti-human KI67 (1:50 550609 BD Biosciences) for proliferation, rabbit anti-human cleaved CASPASE 3 (1:300, 96615 Cell Signaling Technology) for apoptosis, rabbit or goat anti-human SOX9 (1:400 Ab5535, Fisher Scientific

(8) DNA hypomethylation leads to cGAS-induced autoinflammation in the epidermisThe EMBO journalNovember 15, 2021Beck MA, Fischer H, Grabner LM, Groffics T, Winter M, Tangermann S, Meischel T, Zaussinger-Haas B, Wagner P, Fischer C, et al.Loricrin (1:1,000, Covance), p53 (1:50, NCL‐p53‐CM5p, Novocastra), cleaved‐caspase 3 (1:200, CC3, cs‐96615, Cell Signaling), PCNA (1:200, DAKO), DNMT1 (1:200, Abcam, ab188453), CGAS (1:100 Cell Signaling, 31659;

(9) The transcription factor NF-Y participates to stem cell fate decision and regeneration in adult skeletal muscleNature communicationsOctober 14, 2021Rigillo G, Basile V, Belluti S, Ronzio M, Sauta E, Ciarrocchi A, Latella L, Saclier M, Molinari S, Vallarola A, et al.anti-phospho(S139)-H2AX (1:200, #2577 Cell Signaling), rabbit anti-cleaved Caspase- 3 (D175) (1:200, #96615 Cell Signalling). For EdU and Tunel detection, Click-iT EdU cell Proliferation Kit Alexafluor 488 (#C10086

(10) RNA degradation is required for the germ-cell to maternal transition in DrosophilaCurrent BiologyJuly 26, 2021Patrick Blatt, Siu Wah Wong-Deyrup, Alicia McCarthy, Shane Breznak, Matthew D. Hurton, Maitreyi Upadhyay, Benjamin Bennink, Justin Camacho, Miler T. Lee, Prashanth RanganLab,N/A Mouse polyclonal anti-Actin C4,Sigma,MAB1501 Rabbit polyclonal anti-Cleaved Caspase 3,Cell Signalling,#96615 Rabbit polyclonal anti-Egalitarian,Gift from Lehmann Lab,N/A Mouse polyclonal anti-Half pint,Gift

(11) Germacrone Attenuates Hepatic Stellate Cells Activation and Liver Fibrosis via Regulating Multiple Signaling PathwaysFrontiers in pharmacologyJanuary 1, 2021Li Z, Wang Z, Dong F, Shi W, Dai W, Zhao J, Li Q, Fang ZE, Ren L, Liu T, et al.9523), PARP (CST, 9542S), cleaved PARP (CST, 5625S), caspase-3 (CST, 9665S), cleaved caspase-3 (CST, 96615), Bcl-2 (Abcam, ab32124), Bax (Abcam, ab32503), and GAPDH (GeneTex, GTX100118). Cell Culture and Treatment LX-2

(12) Characteristics of Circular RNA Expression Profiles of Porcine Granulosa Cells in Healthy and Atretic Antral FolliclesInternational Journal of Molecular SciencesJuly 23, 2020Li Meng, Katja Teerds, Jian Tao, Hengxi Wei, Marcel Jaklofsky, Zhihong Zhao, Yaodi Liang, Li Li, Chi Chiu Wang, Shouquan Zhangpurchased from Abcam (Cambridge, UK). The antibody against cleaved caspase 3 (cCASP3, lot no 45, cat. no. 96615) was purchased from Cell Signaling Technology (Leiden, The Netherlands). 4.2. Animal and Follicles

(13) Generation of focal mutations and large genomic deletions in the pancreas using inducible in vivo genome editingCarcinogenesisMay 14, 2020Mishra A, Emamgholi F, Erlangga Z, Hartleben B, Unger K, Wolff K, Teichmann U, Kessel M, Woller N, Kühnel F, et al.Denver), anti-Ki67 (17) (1:200, #ab16667; Abcam, Cambridge, UK), anti-cleaved caspase 3 (18) (1:200, #96615; Cell Signalling Technology, Denver). MTAP antibody (1:500, #66706-I-Ig; Proteintech, Rosemont) was

(14) Functionally Distinctive Ptch Receptors Establish Multimodal Hedgehog Signaling in the Tooth Epithelial Stem Cell NicheStem CellsSeptember 1, 2019Binder M, Chmielarz P, Mckinnon PJ, Biggs LC, Thesleff I, Balic A.MAB733-SP",1:500 Lgr5,"Santa Cruz, SC-68580",1:250 pSMAD5/8,"Santa Cruz, SC-12353-R",1:250 Casp3,"Cell Signaling, 96615",1:500 List of antibodies used Abbreviations: Dhh, Desert Hedgehog; Shh, Sonic Hedgehog.

(15) Evaluation of preclinical efficacy of everolimus and pasireotide in thyroid cancer cell lines and xenograft modelsPloS oneJanuary 1, 2019Owonikoko TK, Zhang G, Lallani SB, Chen Z, Martinson DE, Khuri FR, Lonial S, Marcus A, Sun SY.(Cell Signaling, Danvers MA Cat#2976) at 1:1000, cleaved caspase 3 (Cell Signaling, Danvers MA Cat”96615) 1:500; caspase 3 (IMGENEX; USA; Cat #IMG-144A) at 1:500 and somatostatin receptors 1, 2, 3, 4 and 5(Abcam,

(16) A switch in the mode of Wnt signaling orchestrates the formation of germline stem cell differentiation niche in DrosophilaPLOS GeneticsJanuary 25, 2018Maitreyi Upadhyay, Michael Kuna, Sara Tudor, Yesenia Martino Cortez, Prashanth Rangan(1:500, Lehmann Lab), Rb PH3 (1:200, Cell Signaling 97015), and Rb Caspase 3 (1:300, Cell Signaling 96615). Alexa 488 (Molecular Probes), Cy3 and Cy5 (Jackson Labs) conjugated secondary antibodies were used

(17) PSMA-homing dsRNA chimeric protein vector kills prostate cancer cells and activates anti-tumor bystander responsesOncotargetApril 11, 2017Yael Langut, Nufar Edinger, Efrat Flashner-Abramson, Naomi Melamed-Book, Mario Lebendiker, Yael Levi-Kalisman, Shoshana Klein, Alexander Levitzkiwas monitored using anti-PARP (cat#95425), anti-caspase3 (cat#96625) and anti-cleaved caspase-3 (cat#96615) (all from Cell Signaling Technology, Beverly, MA, USA). As an internal control to normalize the amount

(18) Transposon Dysregulation Modulates dWnt4 Signaling to Control Germline Stem Cell Differentiation in DrosophilaPLoS geneticsMarch 1, 2016Upadhyay M, Martino Cortez Y, Wong-Deyrup S, Tavares L, Schowalter S, Flora P, Hill C, Nasrallah MA, Chittur S, Rangan P.Lamin C (1:20, DSHB), Rb H2Av (1:500, Rockland 600-401-914), and Rb Caspase3 (1:300, Cell Signaling 96615). Alexa 488 (Molecular Probes), Cy3 and Cy5 (Jackson Labs) conjugated secondary antibodies were used

(19) L-Aminoacid Oxidase from Bothrops leucurus Venom Induces Nephrotoxicity via Apoptosis and NecrosisPloS oneJanuary 1, 2015Morais IC, Pereira GJ, Orzáez M, Jorge RJ, Bincoletto C, Toyama MH, Monteiro HS, Smaili SS, Pérez-Payá E, Martins AM.conjugated to horseradish peroxidase (Amersham Pharmacia Biotech). The antibodies against Caspase 3 (#96615) and Caspase 7(#9492) were obtained from Cell Signaling, α-tubulin antibody (#T8203) was obtained from

(20) A BAX/BAK and Cyclophilin D-Independent Intrinsic Apoptosis PathwayPLoS ONEJune 12, 2012Sebastián Zamorano, Diego Rojas-Rivera, Fernanda Lisbona, Valentina Parra, Felipe A. Court, Rosario Villegas, Emily H. Cheng, Stanley J. Korsmeyer, Sergio Lavandero, Claudio Hetzcleaved caspase-3 and cleaved caspase-9 immunofluorescence, anti-body were used 1∶250 (#95095 and #96615, Cell signaling). For mitochondrial and cytochrome C (1∶250, BD Pharmigen) colocalization determinations

Be the First to Write a Review!

CR1/CD35 (E8B1Z) Rabbit mAb from Cell Signaling Technology

Questions and Answers

Cell Signaling Technology

Citations (21)

Reviews

CR1/CD35 (E8B1Z) Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map