Complement C4b/d Antibody (16D2) NB200-541 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemComplement C4b/d Antibody (16D2)
CompanyNovus Biologicals, a Bio-Techne Brand
Price $359.00Supplier Page View Company Product Page
Catalog NumberNB200-541
ImmunogenCell-bound C4 of mouse origin
NCBI Gene AliasesCPAMD3, CH, CO4, C4B3, C4B2, C4B1, C4B5, Ss, C4F, C4B_2, C4, C4B12, C4BD
Conjugate/TagUnconjugated
ApplicationsIF/IHC,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunoassay,Immunocytochemistry/ Immunofluorescence
ReactivityMouse
Quantity0.05 mg
TargetComplement C4b/d
IsotypeIgG2a
ClonalityMonoclonal
Clone16D2
HostRat
Format0.2um filtered PBS and 0.1% BSA
Concentration0.1 mg/ml
NCBI Full Gene Namecomplement component 4B (Chido blood group), complement C4B (Chido blood group)
PurityProtein G purified
Antibody TypePrimary Antibodies
StorageStore at 4C

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Antibody cross reacts with C4, C4b and C4d.

Sizes Available: 0.05 mg

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Questions and Answers

Can this antibody be used to study the complement system in mouse models?

Yes, the Complement C4b/d Antibody (16D2) has been utilized in studies examining the complement system in mouse models. For instance, it was employed in immunoblot analyses to detect C4 protein expression in research investigating maternal immune activation and its effects on neurodevelopmental processes in mice ().

Is this antibody effective for detecting C4 in neuroinflammatory conditions?

The Complement C4b/d Antibody has been shown to work effectively in detecting C4 in contexts of neuroinflammation. In a study focused on Toxoplasma infection, the antibody was used to probe for C4 protein levels, contributing to the understanding of complement activation in neuroinflammatory responses ().

What applications is this antibody suitable for in my experiments?

This antibody is suitable for various applications including Western Blotting, Immunoassays, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Immunoprecipitation. Its versatility allows for comprehensive analysis of C4 protein in different experimental setups.

Does this antibody cross-react with other complement proteins?

Yes, the Complement C4b/d Antibody is noted for its specificity and cross-reactivity with C4, C4b, and C4d, making it a valuable tool for studying different forms of the complement protein in various biological contexts.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) IgG hexamers initiate complement-dependent acute lung injuryJournal of Clinical InvestigationJune 3, 2024Simon J. Cleary, Yurim Seo, Jennifer J. Tian, Nicholas Kwaan, David P. Bulkley, Arthur E.H. Bentlage, Gestur Vidarsson, Éric Boilard, Rolf Spirig, James C. Zimring, et al.no. NB200-540), C1qa (clone 4.8, Abcam, catalog no. ab182451), C4b/d (clone 16D2, Novus, catalog no. NB200-541), Scgb1a1 (MilliporeSigma, catalog no. ABS1673), CD41 (clone MWReg30, BioLegend, catalog no. 133902),

(2) PolyI:C Maternal Immune Activation on E9.5 Causes the Deregulation of Microglia and the Complement System in Mice, Leading to Decreased Synaptic Spine DensityInternational Journal of Molecular SciencesMay 17, 2024Shuxin Yan, Le Wang, James Nicholas Samsom, Daniel Ujic, Fang Liu(Abcam, catalog ab200999), rat-monoclonal anti-C4b/d (Novus Biologicals, Centennial, CO, USA, catalog NB200-541), and mouse-monoclonal anti-β-actin (Sigma, Oakville, ON, Canada, catalog A5441). The secondary antibodies

(3) IgG hexamers initiate complement-dependent acute lung injuryJ Clin InvestMarch 26, 2024Simon J. Cleary, Yurim Seo, Jennifer J. Tian, Nicholas Kwaan, David P. Bulkley, Arthur E.H. Bentlage, Gestur Vidarsson, Éric Boilard, Rolf Spirig, James C. Zimring, et al.no. NB200-540), C1qa (clone 4.8, Abcam, catalog no. ab182451), C4b/d (clone 16D2, Novus, catalog no. NB200-541), Scgb1a1 (MilliporeSigma, catalog no. ABS1673), CD41 (clone MWReg30, BioLegend, catalog no. 133902),

(4) Allograft rejection requires STAT5a/b-regulated antiapoptotic activity in T cells but not B cells"Journal of immunology (Baltimore, Md. : 1950)"January 1, 0001"Zhang Y, Kirken RA, Furian L, Janczewska S, Qu X, Hancock WW, Wang M, Tejpal N, Kerman R, Kahan BD, et al."

(5) Synergistic deposition of C4d by complement-activating and non-activating antibodies in cardiac transplantsAmerican journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsJanuary 1, 0001"Murata K, Fox-Talbot K, Qian Z, Takahashi K, Stahl GL, Baldwin WM 3rd, Wasowska BA."

(6) Monoclonal antibodies to complement components without the need of their prior purification. II. Antibodies to mouse C3 and C4HybridomaJanuary 1, 0001"Kremmer E, Thierfelder S, Felber E, Hoffmann-Fezer G, Wasiliu M."

(7) IL-21-producing effector Tfh cells promote B cell alloimmunity in lymph nodes and kidney allograftsJCI insightJanuary 1, 0001"Zhang H, Cavazzoni CB, Podestà MA, Bechu ED, Ralli G, Chandrakar P, Lee JM, Sayin I, Tullius SG, Abdi R, et al."

(8) T cell depletion increases humoral response by favoring T follicular helper cells expansionAmerican journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsJanuary 1, 0001"Gassen RB, Borges TJ, Pérez-Sáez MJ, Zhang H, Al Jurdi A, Llinàs-Mallol L, Aoyama B, Lima M, Pascual J, Sage PT, et al."

(9) Transplantation of Mouse Induced Pluripotent Stem Cell-Derived Podocytes in a Mouse Model of Membranous Nephropathy Attenuates ProteinuriaScientific reportsJanuary 1, 0001"Ahmadi A, Moghadasali R, Ezzatizadeh V, Taghizadeh Z, Nassiri SM, Asghari-Vostikolaee MH, Alikhani M, Hadi F, Rahbarghazi R, Yazdi RS, et al."

(10) Transcriptionally Distinct B Cells Infiltrate Allografts After Kidney TransplantationTransplantationJanuary 1, 0001"Zhang H, Cavazzoni CB, Hanson BL, Bechu ED, Podestà MA, Azzi J, Blazar BR, Chong AS, Kreisel D, Alessandrini A, et al."

(11) Macrophage-derived complement component C4 can restore humoral immunity in C4-deficient mice"Journal of immunology (Baltimore, Md. : 1950)"January 1, 0001"Gadjeva M, Verschoor A, Brockman MA, Jezak H, Shen LM, Knipe DM, Carroll MC."

(12) Immunotherapy targeting the PD-1 pathway alleviates neuroinflammation caused by chronic Toxoplasma infectionScientific reportsJanuary 1, 0001"Xiao J, Li Y, Rowley T, Huang J, Yolken RH, Viscidi RP."cat. ab7260, 1:500, Abcam), C1q (monoclonal, cat. ab71089, 1:1000, Abcam), and C4 (monoclonal, cat. NB200-541, 1:10, Novus Biologicals). Bands were visualized using enhanced chemiluminescence (SuperSignal West Femto Maximum Sensitivity Substrate

(13) Anti-DNA autoreactivity in C4-deficient miceEuropean journal of immunologyJanuary 1, 0001"Paul E, Pozdnyakova OO, Mitchell E, Carroll MC."

(14) Anti-TCRβ mAb induces long-term allograft survival by reducing antigen-reactive T cells and sparing regulatory T cellsAmerican journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsJanuary 1, 0001"Miyahara Y, Khattar M, Schroder PM, Mierzejewska B, Deng R, Han R, Hancock WW, Chen W, Stepkowski SM."

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