Glut1 Antibody NB300-666 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemGlut1 Antibody
CompanyNovus Biologicals, a Bio-Techne Brand
Price $599.00Supplier Page View Company Product Page
Catalog NumberNB300-666
ImmunogenThis Glut1 antibody is made against a synthetic peptide conjugated to KLH, corresponding to amino acids 478-492 of Human Glucose Transporter GLUT1. Peptide was covalently modified. Sequence: PEELFHPLGADSQV
NCBI Gene AliasesDYT17, CSE, GLUT-1, GLUT, GLUT1DS, DYT18, HTLVR, SDCHCN, PED, DYT9, EIG12, GLUT1
FormatTris/Glycine buffer (pH 7.4 - 7.8), HEPES, 0.5% BSA, 30% glycerol
TargetGlut1
ReactivityFish,Human,Mouse,Rat,Zebrafish,Fish - Danio rerio (Zebrafish)
ApplicationsIF/IHC,ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence
Quantity0.1 mg (also 0.025 mg)
HostRabbit
Conjugate/TagUnconjugated
NCBI Full Gene Namesolute carrier family 2 member 1
ClonalityPolyclonal
PurityImmunogen affinity purified
Research AreaCancer, Cardiovascular Biology, Cellular Markers, Diabetes Research, Lipid and Metabolism, Membrane Trafficking and Chaperones, Neuroscience, Plasma Membrane Markers, Signal Transduction
StorageStore at -20C. Avoid freeze-thaw cycles
Molecular Weight54.1 kDa
Antibody TypePrimary Antibodies
IsotypeIgG

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Glucose Transporter GLUT1

Sizes Available: 0.1 mg (also 0.025 mg)

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Questions and Answers

What species does this GLUT1 antibody work with in my experiments?

The GLUT1 antibody has been shown to be reactive with multiple species, including human, mouse, rat, and zebrafish.

Can this antibody be used for immunofluorescence applications?

Yes, the GLUT1 antibody has been utilized in immunofluorescence applications, as indicated by its use in studies such as those by Finicle et al. (2018) and Kim et al. (2016), where it was employed at a dilution of 1:100.

Is there any evidence of this antibody being used in cancer research?

Yes, the GLUT1 antibody has been referenced in cancer research, particularly in studies examining its role in renal cell carcinoma, where it was implicated in the regulation of tumor growth and metabolism (Zhang et al., 2013).

How has this antibody been utilized in flow cytometry?

The GLUT1 antibody has been used in flow cytometry for surface staining, as demonstrated in the study by Harder et al. (2022), where it was part of a panel to analyze B-cell receptor signaling in patients with common variable immunodeficiency.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Sphingolipids inhibit endosomal recycling of nutrient transporters by inactivating ARF6Journal of cell scienceJune 25, 2018Finicle BT, Ramirez MU, Liu G, Selwan EM, McCracken AN, Yu J, Joo Y, Nguyen J, Ou K, Roy SG, et al.Novus Biologicals (MICAL-L1 Cat# H00085377-B01P, IF 1:100 dilution, WB 1:1000 dilution; GLUT1 Cat# NB300-666, IF 1:100 dilution); LI-COR (IRDye 800CW Gt-α-Rb Cat #92632211, WB 1:10,000 dilution; IRDye 680LT Gt-α-Mu

(2) Targeting cancer metabolism by simultaneously disrupting parallel nutrient access pathwaysThe Journal of clinical investigationNovember 1, 2016Kim SM, Roy SG, Chen B, Nguyen TM, McMonigle RJ, McCracken AN, Zhang Y, Kofuji S, Hou J, Selwan E, et al.(catalog 53-1079-42; eBioscience); human 4F2HC (catalog 556077; BD Biosciences); human GLUT1 (catalog NB300-666; Novus Biologicals); LC3 (catalog 4108; Cell Signaling Technology); PIKfyve (catalog 4082; Tocris);

(3) Addressing safety liabilities of TfR bispecific antibodies that cross the blood-brain barrierScience translational medicineMay 1, 2013Couch JA, Yu YJ, Zhang Y, Tarrant JM, Fuji RN, Meilandt WJ, Solanoy H, Tong RK, Hoyte K, Luk W, et al.

(4) Mild Traumatic Brain Injury/Concussion Initiates an Atypical Astrocyte Response Caused by Blood-Brain Barrier DysfunctionJournal of neurotraumaJanuary 1, 0001"George KK, Heithoff BP, Shandra O, Robel S."

(5) Regulation of TFEB and V-ATPases by mTORC1The EMBO journalJanuary 1, 0001"Peña-Llopis S, Vega-Rubin-de-Celis S, Schwartz JC, Wolff NC, Tran TA, Zou L, Xie XJ, Corey DR, Brugarolas J."

(6) Overexpression of factor inhibiting HIF-1 enhances vessel maturation and tumor growth via platelet-derived growth factor-CInternational journal of cancerJanuary 1, 0001"Kuzmanov A, Wielockx B, Rezaei M, Kettelhake A, Breier G."

(7) A feedback loop involving the Phd3 prolyl hydroxylase tunes the mammalian hypoxic response in vivoMolecular and cellular biologyJanuary 1, 0001"Minamishima YA, Moslehi J, Padera RF, Bronson RT, Liao R, Kaelin WG Jr."

(8) Molecular characterization of antibody epitopes of Ehrlichia chaffeensis ankyrin protein 200 and tandem repeat protein 47 and evaluation of synthetic immunodeterminants for serodiagnosis of human monocytotropic ehrlichiosisClinical and vaccine immunology : CVIJanuary 1, 0001"Luo T, Zhang X, Nicholson WL, Zhu B, McBride JW."

(9) Hearts of hypoxia-inducible factor prolyl 4-hydroxylase-2 hypomorphic mice show protection against acute ischemia-reperfusion injuryThe Journal of biological chemistryJanuary 1, 0001"Hyvärinen J, Hassinen IE, Sormunen R, Mäki JM, Kivirikko KI, Koivunen P, Myllyharju J."

(10) Retinal lipid and glucose metabolism dictates angiogenesis through the lipid sensor Ffar1Nature medicineJanuary 1, 0001"Joyal JS, Sun Y, Gantner ML, Shao Z, Evans LP, Saba N, Fredrick T, Burnim S, Kim JS, Patel G, et al."β-Actin (Sigma, A1978), Fibrillarin (Cell Signaling, 2639), Hif1a (Novus, NB100-134), and Glut1 (Novus, NB300-666 and Abcam, Ab652), Glut3 (Millipore, AB1344) and Glut4 (Abcam, ab654) overnight (1:1000 each). After

(11) Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failureBloodJanuary 1, 0001"Minamishima YA, Moslehi J, Bardeesy N, Cullen D, Bronson RT, Kaelin WG Jr."

(12) Systemic long-term inactivation of hypoxia-inducible factor prolyl 4-hydroxylase 2 ameliorates aging-induced changes in mice without affecting their life spanFASEB journal : official publication of the Federation of American Societies for Experimental BiologyJanuary 1, 0001"Laitakari A, Huttunen R, Kuvaja P, Hannuksela P, Szabo Z, Heikkilä M, Kerkelä R, Myllyharju J, Dimova EY, Serpi R, et al."

(13) BAP1 maintains HIF-dependent interferon beta induction to suppress tumor growth in clear cell renal cell carcinomaCancer lettersJanuary 1, 0001"Langbein LE, El Hajjar R, He S, Sementino E, Zhong Z, Jiang W, Leiby BE, Li L, Uzzo RG, Testa JR, et al.",R&D Systems,1:1000 STAT2,A303–512-A,Bethyl Laboratories,1:1000 Bethyl Laboratories,1:1000,HIF2α,A700–003 GLUT-1,NB300–666,Novus Biologicals,1:2000 anti-mouse IgG,62–6520,Invitrogen,1:5000 Invitrogen,1:5000,anti-rabbit IgG,31460 anti-goat

(14) Local angiogenic interplay of Vegfc/d and Vegfa controls brain region-specific emergence of fenestrated capillarieseLifeJanuary 1, 0001"Parab S, Card OA, Chen Q, America M, Buck LD, Quick RE, Horrigan WF, Levkowitz G, Vanhollebeke B, Matsuoka RL."Thermo Fisher Scientific, 1:500), mouse anti-Pgp (#MA1-26528, Thermo Fisher Scientific, 1:100), and rabbit anti-Glut1 (#NB300-666, Novus Biologicals, 1:200). After primary antibody incubation at 4°C overnight, all samples were washed,

(15) The von Hippel-Lindau tumor suppressor protein promotes c-Cbl-independent poly-ubiquitylation and degradation of the activated EGFRPloS oneJanuary 1, 0001"Zhou L, Yang H."Cell Signaling Technology (Boston, MA). Antibodies against HIF2α (NB100-480), HIF1β (NB100-124), GLUT1 (NB300-666) were purchased from Novus Biologicals (Littleton, CO). Antibodies against Cathepsin D (sc-6486), PARP1

(16) Dysregulated PI3K Signaling in B Cells of CVID PatientsCellsJanuary 1, 0001"Harder I, Münchhalfen M, Andrieux G, Boerries M, Grimbacher B, Eibel H, Maccari ME, Ehl S, Wienands J, Jellusova J, et al."anti-rabbit AF647 (cat. #4414) (Cell Signaling Technologies, Frankfurt, Germany); GLUT1 rabbit (cat. #NB300666) (Novus Biologicals, Centennial, CO, USA); 2-NBDG (cat. #N13195) (Invitrogen, Carlsbad, CA, USA); CD19(pY531)

(17) D2HGDH regulates alpha-ketoglutarate levels and dioxygenase function by modulating IDH2Nature communicationsJanuary 1, 0001"Lin AP, Abbas S, Kim SW, Ortega M, Bouamar H, Escobedo Y, Varadarajan P, Qin Y, Sudderth J, Schulz E, et al."Biosciences, #61095), HIF1α-hydroxyproline (Pro-402; Millipore, #07-1585), GLUT1 (Novus Biologicals, # NB300-666), IDH1 (Cell Signaling Technology, #8137), IDH2 (Abcam, #ab55271), β-actin (Sigma-Aldrich, #A2228).

(18) Oxidative stress-induced alterations in retinal glucose metabolism in Retinitis PigmentosaFree radical biology & medicineJanuary 1, 0001"Kanan Y, Hackett SF, Taneja K, Khan M, Campochiaro PA."31586055, TIGAR (1:1000, 22136-1-AP, Proteintech) validated by Proteintech on western blots, Glut1 (1:1000, NB300-666, Novus) PMID: 30332638 and GRK1 (1:1000, PA5-13725, ThermoFischer Scientific) PMID: 32019921. Secondary

(19) Transformation by the (R)-enantiomer of 2-hydroxyglutarate linked to EGLN activationNatureJanuary 1, 0001"Koivunen P, Lee S, Duncan CG, Lopez G, Lu G, Ramkissoon S, Losman JA, Joensuu P, Bergmann U, Gross S, et al."Product), anti-EglN1(PHD2) monoclonal antibody (D31E11, Cell Signaling), anti-GLUT-1 polyclonal antibody (NB300-666, Novus Biologicals), mouse monoclonal anti-tubulin (B-512, Sigma-Aldrich) or anti-vinculin Nature.

(20) A cell-penetrating peptide suppresses the hypoxia inducible factor-1 function by binding to the helix-loop-helix domain of the aryl hydrocarbon receptor nuclear translocatorChemico-biological interactionsJanuary 1, 0001"Wang Y, Thompson JD, Chan WK."rabbit IgG (sc-25599) were purchased from Santa Cruz Biotechonology. Anti-Glut1 polyclonal rabbit IgG (NB300–666) was purchased from Novus Biologicals (Littleton, CO). Anti-HIF1α monoclonal rabbit IgG (2015-1) was

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Glut1 Antibody

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Glut1 Antibody

Glut1 Antibody from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Questions and Answers

Novus Biologicals, a Bio-Techne Brand

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Glut1 Antibody from Novus Biologicals, a Bio-Techne Brand

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