Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Action potentials were evoked with depolarizing current pulses. (A) Neuron from colonic specimen of non-treated patient fired 1 action potential in response to a depolarizing current. (A') Intracellular injection of carboxyfluorescein during recording confirmed Dogiel type I, uniaxonal morphology. (A") IHC demonstrated neuron was Hu-immunoreactive but did not contain nNOS immunoreactivity. (B) Neuron from a chemotherapy-treated patient fired multiple action potentials in response to a smaller depolarising current pulse. (B') Intracellular injection of carboxyfluorescein confirmed neuronal morphology. (B") This neuron was Hu-immunoreactive, but not nNOS-immunoreactive, scale bar = 100 um.

Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Action potentials were evoked with depolarizing current pulses. (A) Neuron from colonic specimen of non-treated patient fired 1 action potential in response to a depolarizing current. (A') Intracellular injection of carboxyfluorescein during recording confirmed Dogiel type I, uniaxonal morphology. (A") IHC demonstrated neuron was Hu-immunoreactive but did not contain nNOS immunoreactivity. (B) Neuron from a chemotherapy-treated patient fired multiple action potentials in response to a smaller depolarising current pulse. (B') Intracellular injection of carboxyfluorescein confirmed neuronal morphology. (B") This neuron was Hu-immunoreactive, but not nNOS-immunoreactive, scale bar = 100 um.

Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Action potentials were evoked with depolarizing current pulses. (A) Neuron from colonic specimen of non-treated patient fired 1 action potential in response to a depolarizing current. (A') Intracellular injection of carboxyfluorescein during recording confirmed Dogiel type I, uniaxonal morphology. (A") IHC demonstrated neuron was Hu-immunoreactive but did not contain nNOS immunoreactivity. (B) Neuron from a chemotherapy-treated patient fired multiple action potentials in response to a smaller depolarising current pulse. (B') Intracellular injection of carboxyfluorescein confirmed neuronal morphology. (B") This neuron was Hu-immunoreactive, but not nNOS-immunoreactive, scale bar = 100 um.

Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Action potentials were evoked with depolarizing current pulses. (A) Neuron from colonic specimen of non-treated patient fired 1 action potential in response to a depolarizing current. (A') Intracellular injection of carboxyfluorescein during recording confirmed Dogiel type I, uniaxonal morphology. (A") IHC demonstrated neuron was Hu-immunoreactive but did not contain nNOS immunoreactivity. (B) Neuron from a chemotherapy-treated patient fired multiple action potentials in response to a smaller depolarising current pulse. (B') Intracellular injection of carboxyfluorescein confirmed neuronal morphology. (B") This neuron was Hu-immunoreactive, but not nNOS-immunoreactive, scale bar = 100 um.