Western blot analysis of extracts of 293T cells, using Phospho-POLR2A-S2 pAb (TA380137) at 1:1000 dilution or POLR2A antibody (A2107).293T cells were treated by CIP(20uL/400ul) at 37‚ÑÉ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 1s.

Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug Phospho-POLR2A-S2 pAb (TA380137). Western blot was performed from the immunoprecipitate using Phospho-POLR2A-S2 pAb (TA380137) at a dilition of 1:1000. MCF7 cells were treated by nocodazole (50 ng/mL) at 37‚ÑÉ for 20 hours.