Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Chk2-S516 pAb (TA374729) at 1:1000 dilution or Chk2 antibody (A0466).NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 10s.