Flow-cytometry using anti-PD-1 antibody RMP1-14 (TA386122) Staining of murine splenocytes (unstimulated and 3-day Con A stimulated) with the rabbit-chimeric version of RMP1-14 (TA386122) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody. Con A stimulation induces PD-1 expression as evident by the increased fluorescence signal.