Western blot analysis of extracts of HeLa cells, using Phospho-SMC1-S957 pAb (TA381769) at 1:1000 dilution or SMC1A antibody (A7008).HeLa cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 3s.

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Phospho-SMC1-S957 antibody (TA381769). Western blot was performed from the immunoprecipitate using Phospho-SMC1-S957 antibody (TA381769) at a dilition of 1:1000.