Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) (-) or SignalSilence® PFKP siRNA (+), using PFKP (D2E5) Rabbit mAb and β-Actin (D6A8) Rabbit mAb.

Immunoprecipitation of PFKP from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control (lane 2) or PFKP (D2E5) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PFKP (D2E5) Rabbit mAb.

Confocal immunofluorescent analysis of 293 cells, transfected with SignalSilence® Control siRNA (Unconjugated) (left) or a PFKP-specific siRNA (right), using PFKP (D2E5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).