Fig 1: SCFAs antagonize NFκB/MAPK-mediated ISGs signaling. HTMCs (n = 3) were pretreated with PBA, NaB, or NaAc followed by ZIKV (Z) (PRVABC59 strain, MOI: 1) infection for 48 h. Mock-treated cells were used as controls. (A) The cell lysates from mock, ZIKV-infected, and drug-treated cells were subjected to western blotting for the NFκB, ERK1/2, p38, STAT1, STAT2, STST3, RIG-I, IRF3, and IFIT2 pathways. (B) Densitometric analysis of the immunoblots was performed using ImageJ. The bar graph represents means ± SD from three biological replicates. *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001 (one-way ANOVA).
from Cell Signaling Technology for IFIT2 Antibody