pIgR Antibody AF2800 from R&D Systems, a Bio-Techne Brand

Product Specs
ItempIgR Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF2800
ApplicationsELISA Development,ELISA Development (Capture),IHC Paraffin-embedded,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Blockade of Receptor-ligand Interaction,Immunocytochemistry,ELISA Capture
Quantity100 ug (also 25 ug)
IsotypeIgG
TargetpIgR
ReactivityHuman,Mouse,Plant,Porcine,Transgenic Mouse
HostGoat
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
NCBI Full Gene Namepolymeric immunoglobulin receptor
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse pIgR, Lys19-Lys645, Accession # O70570
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse pIgR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human pIgR is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for Western blotting in my experiments?

Yes, the pIgR antibody has been shown to work effectively in Western blot applications, as evidenced by its use in studies such as the one by Azzam et al. (2017), where it was employed to detect pIgR in mouse tissue samples.

Is this antibody suitable for immunohistochemistry applications?

The pIgR antibody has been utilized in immunohistochemistry, as demonstrated in the study by Hu et al. (2022), where it was applied to analyze tissue sections, indicating its effectiveness in this application.

What species can this antibody detect, and is there any cross-reactivity?

The pIgR antibody is reactive with mouse, human, and plant species. It exhibits approximately 5% cross-reactivity with recombinant human pIgR, making it suitable for studies involving these species.

How should I store this antibody after reconstitution?

After reconstitution, the pIgR antibody should be stored under sterile conditions at -20 to -70 °C for up to six months. It can also be kept at 2 to 8 °C for one month, but repeated freeze-thaw cycles should be avoided to maintain its stability.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Maternal supplementation with Lactobacillus reuteri enhances colostrum sIgA secretion and immune function of offspringAnimal MicrobiomeMarch 17, 2026Qian Xie, Pengyi Tian, Qing Duanmu, Mei Yang, Chen Zhang, Jing Wang, Bi E. Tanpolyvinylidene difluoride membranes, followed by incubation with primary and secondary antibodies (AF2800, HAF017, R&D Systems, America), and visualized using chemiluminescent reagent (P10300, NCM Biotech,

(2) Development of a cyst-targeted therapy for polycystic kidney disease using an antagonistic dimeric IgA monoclonal antibody against cMETCell reports. MedicineSeptember 16, 2025Schimmel MF, Bourgeois BC, Spindt AK, Patel SA, Chin T, Cornick GE, Liu Y, Weimbs T.A18789; RRID: AB_2535566 beta-actin,Sigma-Aldrich,Cat# A5441; RRID: AB_476744 pIgR,R&D Systems,Cat#AF2800; RRID: AB_2283871 AQP1 (B-11),Santa Cruz Biotechnology,Cat#sc-25287; RRID: AB_626694 mouse Ki67,EMD Millipore

(3) Secretory IgA in breast milk protects against asthma through modulation of the gut microbiotaCell reportsOctober 22, 2024Donald K, Serapio-Palacios A, Gerbec Z, Bozorgmehr T, Holani R, Cruz AR, Schnupf P, Finlay BB.

(4) Intestinal epithelium dysfunctions cause IgA deposition in the kidney glomeruli of intestine-specific Ap1m2-deficient miceEBioMedicineAugust 1, 2024Kinashi Y, Tanaka K, Kimura S, Hirota M, Komiyama S, Shindo T, Hashiguchi A, Takahashi D, Shibata S, Karaki SI, et al.

(5) Single-cell spatial transcriptomics reveals a dynamic control of metabolic zonation and liver regeneration by endothelial cell Wnt2 and Wnt9bCell reports. MedicineOctober 18, 2022Hu S, Liu S, Bian Y, Poddar M, Singh S, Cao C, McGaughey J, Bell A, Blazer LL, Adams JJ, et al.Cyclin D1 (Abcam, ab134175, 1:200), RGN (Santa Cruz Biotechnology, sc-390098, 1:100), PIGR (R&D Systems, AF2800, 1:100) for 1 h at room temperature. Samples were washed with PBS three times and incubated with the

(6) Porcine Milk-Derived Small Extracellular Vesicles Promote Intestinal Immunoglobulin Production through pIgRAnimals : an open access journal from MDPIMay 24, 2021Zeng B, Wang H, Luo J, Xie M, Zhao Z, Chen X, Wang D, Sun J, Xi Q, Chen T, et al.

(7) Lung immunoglobulin A immunity dysregulation in cystic fibrosisEBioMedicineOctober 1, 2020Collin AM, Lecocq M, Noel S, Detry B, Carlier FM, Aboubakar Nana F, Bouzin C, Leal T, Vermeersch M, De Rose V, et al.

(8) Fecal IgA Levels Are Determined by Strain-Level Differences in Bacteroides ovatus and Are Modifiable by Gut Microbiota ManipulationCell host & microbeMarch 11, 2020Yang C, Mogno I, Contijoch EJ, Borgerding JN, Aggarwala V, Li Z, Siu S, Grasset EK, Helmus DS, Dubinsky MC, et al.(1/300 dilution; eBioscience, clone mA-6E1), goat anti-mouse pIgR (1/500 dilution; R&D Systems, cat #: AF2800). Slides were washed in PBS for three times, incubated with Alexa Fluor®-conjugated species-specific

(9) Single-cell mapping of the thymic stroma identifies IL-25-producing tuft epithelial cellsNatureJuly 1, 2018Bornstein C, Nevo S, Giladi A, Kadouri N, Pouzolles M, Gerbe F, David E, Machado A, Chuprin A, Tóth B, et al.

(10) Pulmonary Regnase-1 orchestrates the interplay of epithelium and adaptive immune systems to protect against pneumoniaMucosal immunologyJuly 1, 2018Nakatsuka Y, Vandenbon A, Mino T, Yoshinaga M, Uehata T, Cui X, Sato A, Tsujimura T, Suzuki Y, Sato A, et al.

(11) Loss of CD14 leads to disturbed epithelial-B cell crosstalk and impairment of the intestinal barrier after E. coli Nissle monoassociationScientific reportsJanuary 15, 2018Basic M, Buettner M, Keubler LM, Smoczek A, Bruesch I, Buchheister S, Bleich A.

(12) Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfacesJCI insightAugust 17, 2017Azzam KM, Madenspacher JH, Cain DW, Lai L, Gowdy KM, Rai P, Janardhan K, Clayton N, Cunningham W, Jensen H, et al.A3854), goat anti-IgA HRP (1:1,000; Abcam, ab97235), or goat anti-pIgR (1:1,000; R&D Systems/Bio-techne, AF2800). Membranes were then washed and the latter target exposed (60 minutes) to 1:5,000 HRP-conjugated secondary

(13) Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgAScientific reportsApril 3, 2017Nakanishi K, Morikane S, Ichikawa S, Kurohane K, Niwa Y, Akimoto Y, Matsubara S, Kawakami H, Kobayashi H, Imai Y.

(14) Contribution of mucus concentration and secreted mucins Muc5ac and Muc5b to the pathogenesis of muco-obstructive lung diseaseMucosal immunologyMarch 1, 2017Livraghi-Butrico A, Grubb BR, Wilkinson KJ, Volmer AS, Burns KA, Evans CM, O'Neal WK, Boucher RC.in PBS-T, for 1 hr at RT. Primary antibodies and isotype control (goat anti-mouse pIgR R&D Systems AF2800 and goat IgG Jackson ImmunoResearch 0.2 mg/ml) were diluted in 5% NDS in PBST and incubated over night

(15) Exploitation of the Polymeric Immunoglobulin Receptor for Antibody Targeting to Renal Cyst Lumens in Polycystic Kidney DiseaseThe Journal of biological chemistryJune 19, 2015Olsan EE, Matsushita T, Rezaei M, Weimbs T.

(16) Vertically transmitted faecal IgA levels determine extra-chromosomal phenotypic variationNatureMay 7, 2015Moon C, Baldridge MT, Wallace MA, D CA, Burnham, Virgin HW, Stappenbeck TS.antibody at 4°C overnight. Primary antibodies include: goat anti-mouse pIgR (1/500, R&D Systems, catalog #AF2800) and goat anti-mouse IgA-AlexaFluor488 (1/200, Serotec, catalog #STAR137F). The slides were rinsed three

(17) Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosisMucosal immunologyJuly 1, 2014Moon C, VanDussen KL, Miyoshi H, Stappenbeck TS.

(18) Aldehyde dehydrogenase activity enriches for proximal airway basal stem cells and promotes their proliferationStem cells and developmentMarch 15, 2014Hegab AE, Ha VL, Bisht B, Darmawan DO, Ooi AT, Zhang KX, Paul MK, Kim YS, Gilbert JL, Attiga YS, et al.

(19) IL-25 improves IgA levels during parenteral nutrition through the JAK-STAT pathwayAnnals of surgeryDecember 1, 2013Heneghan AF, Pierre JF, Kudsk KA.room temperature. Sections were incubated with pIgR primary antibody (1:100, Goat anti-mouse polyclonal antibody, AF2800, R&D Systems, Minneapolis, MN) in 1% BSA with PBS for 1 hour at room temperature. Sections were rinsed

(20) Targeted deletion of MyD88 in intestinal epithelial cells results in compromised antibacterial immunity associated with downregulation of polymeric immunoglobulin receptor, mucin-2, and antibacterial peptidesMucosal immunologySeptember 1, 2012Frantz AL, Rogier EW, Weber CR, Shen L, Cohen DA, Fenton LA, Bruno ME, Kaetzel CS.

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pIgR Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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pIgR Antibody from R&D Systems, a Bio-Techne Brand

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