Western blot analysis of TGF beta 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500496, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-3 cell lysate Lane 2: A549 cell lysate Lane 3: U87 cell lysate Lane 4: Hela cell lysate Lane 5: K562 cell lysate