Fig 1: Cell senescence was induced in kidney IRI. (A) Representative images of H&E, Masson, and IHC staining of cellular senescence-associated markers (p53, p21, and p16) of human kidney biopsies (T0-B: time-zero biopsies, CIA: chronically injured allografts). Scale bars, 100 μm. Right: statistical analysis of Masson, and IHC staining. (B) Representative images of H&E (200 μm), Masson (200 μm), SABG (50 μm), staining, and quantifications of mice at different times. (C) The serum concentrations of Scr and BUN at different times. (D) Representative bands and statistical analysis of protein expression of mice kidneys at different times. BUN, blood urea nitrogen; CIA, chronically injured allografts; H&E, hematoxylin and eosin; IHC, immunohistochemical; IRI, ischemia/reperfusion injury; SABG, senescence-associated beta-galactosidase; Scr, serum creatinine; T0-B, time-zero biopsies.