Western blot analysis of PEX11B on different lysates with Rabbit anti-PEX11B antibody (HA721124) at 1/1,000 dilution. Lane 1: Human lung tissue lysate, 20 µg/Lane Lane 2: MCF-7 cell lysate, 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 25 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721124) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.