Western blot analysis of SF3A3 on different lysates with Rabbit anti-SF3A3 antibody (HA721114) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse liver tissue lysate Lane 6: Rat liver tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721114) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.