Fig 2: Expression of PKC-Δ and caspase-3 proteins in treated and untreated Chang cell line. Cells were treated with isolated PEs from Jatropha meal and PMA at the CC50 concentration incubated for 24 h. Equal amounts of total cellular protein of treated and control cells were subjected to Western blot analyses for PKC-Δ, caspase-3 and GAPDH protein expression. All values represent mean ± standard error from three independent experiments, ***p < 0.001 and **p < 0.01 indicate significant difference compared to the untreated control.

Fig 3: Expression of PKC-δ and Caspase-3 proteins in treated and un-treated MCF-7 cell line. Cells were treated with isolated PEs from Jatropha meal and PMA at the IC50 concentration incubated for 24 h. Equal amounts of total cellular protein of treated and un-treated cells were subjected to Western blot analyses for PKC-δ, Caspase-3 and GAPDH protein expression. All values represent mean ± standard error from three independent experiments, *** p ≤ 0.001 and ** p ≤ 0.01 indicate significant difference compared to the untreated control.

Fig 4: Expression of PKC-Δ and caspase-3 proteins in treated and untreated Vero cell line. Cells were treated with isolated PEs from Jatropha meal and PMA at the CC50 concentration incubated for 24 h. Equal amounts of total cellular protein of treated and control cells were subjected to Western blot analyses for PKC-Δ, caspase-3 and GAPDH protein expression. All values represent mean ± standard error from three independent experiments, ***p < 0.001 and **p < 0.01indicate significant difference compared to the untreated control.

Fig 5: Expression of PKC-δ and Caspase-3 proteins in treated and un-treated HeLa cell line. Cells were treated with isolated PEs from Jatropha meal and PMA at the IC50 concentration incubated for 24 h. Equal amounts of total cellular protein of treated and un-treated cells were subjected to Western blot analyses for PKC-δ, Caspase-3 and GAPDH protein expression. All values represent mean ± standard error from three independent experiments, *** p ≤ 0.001 and ** p ≤ 0.01indicate significant difference compared to the untreated control.