Fig 2: Characterisation of ovine ileum organoids by immunofluorescence confocal microscopy at seven days of in vitro culture. (A) Representative Z-stack images of part of an individual branched ileum organoid with a closed luminal space and an internal F-actin-expressing brush border. Red = F-actin and blue = Hoechst (nuclei). (B) Representative images of abomasum organoids probed for either the cell proliferation marker Ki67, or the epithelial cell markers EpCAM, villin and pan-cytokeratin (all green). F-actin (red) and Hoechst (blue). Scale bars = 10 µm.

Fig 3: Characterisation of ovine abomasum organoids by immunofluorescence confocal microscopy at seven days of in vitro culture. (A) Representative Z-stack images of an individual abomasum organoid with a closed luminal space and an internal F-actin-expressing brush border. Red = F-actin and blue = Hoechst (nuclei). (B) Representative images of abomasum organoids probed for either the cell proliferation marker Ki67, or the epithelial cell markers EpCAM, villin and pan-cytokeratin (all green). F-actin (red) and Hoechst (blue). Scale bars = 10 µm.