Immunohistochemistry (Paraffin) analysis of RHBDF2 using RHBDF2 Polyclonal Antibody (Product # PA5-121461) at a dilution of 1:200 in human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.