CHL-1/L1CAM-2 Antibody AF2147 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCHL-1/L1CAM-2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF2147
ApplicationsFlow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Frozen,Mass Spectrometry,Simple Western,Neutralization,Immunocytochemistry,Cell Culture
ReactivityHuman,Mouse,Rat,Transgenic Mouse
TargetCHL-1/L1CAM-2
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
IsotypeIgG
Conjugate/TagUnconjugated
HostGoat
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse CHL-1/L1CAM-2, Ala25-Gln1043 (Leu227-Gln242 del, Ala243Ser), Accession # BAC30699
ConcentrationLYOPH
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namecell adhesion molecule L1 like
NCBI Gene AliasesCALL, L1CAM2
PurityAntigen Affinity-purified

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse CHL-1/L1CAM-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human CHL-1 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Glial-to-mesenchymal transition of tumor Schwann cells drives the genetic burden in MPNSTs from neurofibromatosis type 1 mouse modelScience advancesNovember 14, 2025Radomska KJ, Onfroy A, Lecerf L, Job B, Beaude A, Sanz LS, El Jalkh T, Thieffry D, Charnay P, Wolkenstein P, et al.

(2) RAB35 is required for murine hippocampal development and functions by regulating neuronal cell distributionCommunications biologyApril 21, 2023Maejima I, Hara T, Tsukamoto S, Koizumi H, Kawauchi T, Akuzawa T, Hirai R, Kobayashi H, Isobe I, Emoto K, et al.Merck-Millipore, MAB374), 1:1000 contactin-2/TAG-1 (goat; R&D systems, AF4439), 1:1000 CHL1 (goat; R&D systems, AF2147), and 1:1000 N-cadherin [32/N-cadherin] (rabbit; BD, 610920). The following primary antibodies were

(3) Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesityNature communicationsNovember 25, 2022Duregotti E, Reumiller CM, Mayr U, Hasman M, Schmidt LE, Burnap SA, Theofilatos K, Barallobre-Barreiro J, Beran A, Grandoch M, et al.

(4) Interplay in neural functions of cell adhesion molecule close homolog of L1 (CHL1) and Programmed Cell Death 6 (PDCD6)FASEB bioAdvancesJanuary 1, 2022Loers G, Theis T, Baixia Hao H, Kleene R, Arsha S, Samuel N, Arsha N, Young W, Schachner M.numbers ORG 679 Morph and ORG 1022). 2.2 Antibodies and chemical reagents Polyclonal goat antibody (#AF2147) against CHL1 was purchased from R&D Systems. Rabbit polyclonal PDCD6 antibody (#12303‐1‐AP) was purchased

(5) Spatiotemporal processing of neural cell adhesion molecules 1 and 2 by BACE1 in vivoThe Journal of biological chemistryJanuary 1, 2021Kim W, Watanabe H, Lomoio S, Tesco G.Cell Signaling), anti-BACE1 (rabbit mAb, 1:1500; D10E5; Cell signaling); anti-CHL1 (goat pAb, 1:2000; AF2147; R&D systems); anti-calnexin (mouse mAb, 1:1000; 610523; BD biosciences); anti-GAPDH (mouse mAb, 1:10,000;

(6) BACE1 partial deletion induces synaptic plasticity deficit in adult miceScientific reportsDecember 27, 2019Lombardo S, Chiacchiaretta M, Tarr A, Kim W, Cao T, Sigal G, Rosahl TW, Xia W, Haydon PG, Kennedy ME, et al.C1/6.1; BioLegend); goat polyclonal anti-N-terminal CHL1 antibody (for CHL1-FL and CHL1-NTF) (1:1000; AF2147; R&D Systems); mouse monoclonal anti-GAPDH (1:10,000; MAP374; Millipore); mouse monoclonal anti-β-tubulin

(7) Axonal Growth of Midbrain Dopamine Neurons is Modulated by the Cell Adhesion Molecule ALCAM Through Trans-Heterophilic Interactions with L1cam, Chl1, and SemaphorinsThe Journal of neuroscience : the official journal of the Society for NeuroscienceAugust 21, 2019Bye CR, Rytova V, Alsanie WF, Parish CL, Thompson LH.

(8) Close Homolog of L1 Regulates Dendritic Spine Density in the Mouse Cerebral Cortex Through Semaphorin 3BThe Journal of neuroscience : the official journal of the Society for NeuroscienceAugust 7, 2019Mohan V, Wade SD, Sullivan CS, Kasten MR, Sweetman C, Stewart R, Truong Y, Schachner M, Manis PB, Maness PF.Sections or cultures were incubated overnight with goat polyclonal anti-CHL1 (R&D Systems catalog #AF2147, RRID:AB_2079332), rabbit polyclonal antiNrCAM (Abcam catalog #24344, RRID:AB_448024), rabbit polyclonal anti-Sema3B

(9) Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer targetCancer scienceAugust 1, 2019Kotani N, Yamaguchi A, Ohnishi T, Kuwahara R, Nakano T, Nakano Y, Ida Y, Murakoshi T, Honke K.at room temperature and then treated with either 5 μg/mL of HRP‐conjugated anti–mouse CHL1 antibody (AF2147; R&D systems) and anti–human CHL1 antibody (MAB2126; R&D systems) or 4 μg/mL of HRP‐conjugated CTxB

(10) Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAMNeuronApril 17, 2019Tai Y, Gallo NB, Wang M, Yu JR, Van Aelst L.ab24704), anti-TAG-1 (goat pAb, 1:1000, R&D Systems AF4439), anti-CHL1 (goat pAb, 1:1000, R&D Systems AF2147), anti-γ-tubulin (mouse mAb IgG1, 1:5000, Sigma-Aldrich T6557), anti-β-actin (mouse mAb IgG1, 1:9000,

(11) Axonal organization defects in the hippocampus of adult conditional BACE1 knockout miceScience Translational MedicineSeptember 19, 2018Ming-Hsuan Ou-Yang, Jonathan E. Kurz, Toshihiro Nomura, Jelena Popovic, Tharinda W. Rajapaksha, Hongxin Dong, Anis Contractor, Dane M. Chetkovich, Warren G. Tourtellotte, Robert Vassar(ThermoFisher, MA1–82088), rabbit anti-Calbindin (Cell Signaling, #2173), goat anti-CHL1 (R&D systems, AF2147), rat anti-Myelin Basic Protein (Abcam, ab7349), rabbit anti-Neuregulin type III (Millipore, AB5551),

(12) Cell Adhesion Molecule Close Homolog of L1 (CHL1) Guides the Regrowth of Regenerating Motor Axons and Regulates Synaptic Coverage of Motor NeuronsFrontiers in Molecular NeuroscienceMay 24, 2018Daria Guseva, Igor Jakovcevski, Andrey Irintchev, Iryna Leshchyns’ka, Vladimir Sytnyk, Evgeni Ponimaskin, Melitta Schachner

(13) BACE1 elevation engendered by GGA3 deletion increases β-amyloid pathology in association with APP elevation and decreased CHL1 processing in 5XFAD miceMolecular neurodegenerationFebruary 2, 2018Kim W, Ma L, Lomoio S, Willen R, Lombardo S, Dong J, Haydon PG, Tesco G.Sigma Aldrich); goat polyclonal anti-N-terminal CHL1 antibody to detect mouse CHL1_βNTF and CHL1_FL (1:1000; AF2147; R&D Systems); rat monoclonal anti-N-terminal CHL1 antibody to detect human CHL1_βNTF and CHL1_FL (1:2000;

(14) Proteolytic cleavage of transmembrane cell adhesion molecule L1 by extracellular matrix molecule Reelin is important for mouse brain developmentScientific reportsNovember 10, 2017Lutz D, Sharaf A, Drexler D, Kataria H, Wolters-Eisfeld G, Brunne B, Kleene R, Loers G, Frotscher M, Schachner M.(ab16046), Tbr1 (ab31940), and Cutl1 (ab140042) were from Abcam (Cambridge, UK). Goat CHL1 antibody (AF2147) was from R&D Systems. Production of rabbit NCAM antibodies and of L1-Fc comprising the extracellular

(15) Aβ-dependent reduction of NCAM2-mediated synaptic adhesion contributes to synapse loss in Alzheimer's diseaseNature communicationsNovember 27, 2015Leshchyns'ka I, Liew HT, Shepherd C, Halliday GM, Stevens CH, Ke YD, Ittner LM, Sytnyk V.immobilize NCAM2 for ELISA (10 μg ml−1). Goat antibodies against the extracellular domain of CHL1 (AF2147; R&D systems) were used for WB (1 μg ml−1); goat polyclonal and mouse monoclonal antibodies against

(16) Antagonistic Effects of BACE1 and APH1B-γ-Secretase Control Axonal Guidance by Regulating Growth Cone CollapseCell reportsSeptember 1, 2015Barão S, Gärtner A, Leyva-Díaz E, Demyanenko G, Munck S, Vanhoutvin T, Zhou L, Schachner M, López-Bendito G, Maness PF, et al.by western blot to detect full-length or C-terminal fragments of the proteins of interest (full-length-CHL1 [AF2147; R&D Systems], CHL1CTFs [antibody generated by Thermo Fisher Scientific using peptide DGSFIGAYTGAKEKGSVE],

(17) Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitorsProceedings of the National Academy of Sciences of the United States of AmericaApril 14, 2015Bye CR, Jönsson ME, Björklund A, Parish CL, Thompson LH.

(18) An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's diseaseEMBO molecular medicineFebruary 1, 2015Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido TC, Nakano M, Yamaguchi Y, Hashimoto Y, Staufenbiel M, et al.anti-MAP2 (sc-20172) from Santa Cruz Biotechnology; anti-GFAP (13-0300) from Life Technologies; anti-CHL1 (AF2147) and anti-contactin-2 (AF4439) from R&D systems; and anti-Iba1 (019-197419) from Wako. Biotinylated

(19) Endothelial VEGF sculpts cortical cytoarchitectureThe Journal of neuroscience : the official journal of the Society for NeuroscienceSeptember 11, 2013Li S, Haigh K, Haigh JJ, Vasudevan A.mouse monoclonal antibody to RC2 (1:5, MAB5740, Millipore), goat polyclonal antibody to CHL1 (1:50, AF2147, R&D Systems), rat monoclonal antibody to CD31/PECAM-1 (1:50, 550274, BD Biosciences PharMingen), and

(20) β-Site amyloid precursor protein (APP)-cleaving enzyme 1 (BACE1)-deficient mice exhibit a close homolog of L1 (CHL1) loss-of-function phenotype involving axon guidance defectsThe Journal of biological chemistryNovember 9, 2012Hitt B, Riordan SM, Kukreja L, Eimer WA, Rajapaksha TW, Vassar R.

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CHL-1/L1CAM-2 Antibody

CHL-1/L1CAM-2 Antibody

CHL-1/L1CAM-2 Antibody

CHL-1/L1CAM-2 Antibody

CHL-1/L1CAM-2 Antibody

CHL-1/L1CAM-2 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (29)

Reviews

CHL-1/L1CAM-2 Antibody from R&D Systems, a Bio-Techne Brand

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