Fig 1: Representative images of immunofluorescent Gli2 staining in motor neurons of the lumbar spinal cord in WT and mSOD mice. For all age groups of WT mice, Gli2 staining (yellow arrows) was observed predominantly in the nucleus and less in the cytoplasm (a, c, and e). However, Gli2 staining (yellow arrows) was redistributed from the nucleus to the cytoplasm in mSOD mice (b, d, and f). The intensity of Gli2 staining was also reduced compared with age-matched WT controls. Scale bar=50 µm. mSOD, mutant superoxide dismutase; WT, wild type.