CD206/MRC1 (E6T5J) XP ® Rabbit mAb 24595T from Cell Signaling Technology

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Molecular therapy. Nucleic acids
September 03 2021

Fig 1: Naringin regulated LPS-stimulated macrophage polarization via stimulating PPARγ and its downstream miRNA expression(A) An MTT assay was used to assess the toxicity of naringin with designated doses. (B) The mRNA levels of TNF-α, IL-1β, and IL-6 in BMDMs were measured by quantitative real-time PCR. (C) The secretion levels of TNF-α, IL-1β, and IL-6 in culture medium of BMDMs were measured by ELISA. (D) M1 macrophages (F4/80+CD86+) and M2 macrophages (F4/80+CD206+) in LPS-stimulated BMDMs with or without naringin administration (100 μM) were examined by flow cytometry. (E and F) The mRNA levels of M1 markers iNOS and CD86 (E) and M2 markers Arg-1 and IL-10 (F) were measured by quantitative real-time PCR. (G) The protein level of PPARγ was measured by western blotting with quantitative analysis. (H) The expression levels of miRNAs associated with macrophage polarization were detected by quantitative real-time PCR. Data represent the mean ± SD of three experiments performed in triplicate independently. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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Frontiers in immunology
January 01 2022

Fig 2: (A) Expression level (IHC quantification) of RIPK1, RIPK3, MLKL (necroptosis core mediators), NRGPI oncogenes (SERPINE1, GPX3, GRP, FCN1, CYTL1, CNTN1, PLCL1, and APOD), and markers of WNT signaling pathway (WNT2B, WNT9A), TGF-β signaling pathway (TGFB1, TGFB3), and macrophage (CD63, CD206, CD163) in the clinical samples (n = 4) of stomach adenocarcinoma. The scattered dots represent the IHC score of each individual sample. The thick middle lines represent the median value and error bars indicate the standard deviation. The bottom and top of the boxes are the 25th and 75th percentiles (interquartile range), respectively. (B) A correlation matrix illustrating Pearson’s correlation coefficients indicating the relationship among expression levels of RIPK1, RIPK3, MLKL (necroptosis core mediators), NRGPI oncogenes (SERPINE1, GPX3, GRP, FCN1, CYTL1, CNTN1, PLCL1, and APOD), and markers of WNT signaling pathway (WNT2B, WNT9A), TGF-β signaling pathway (TGFB1, TGFB3), and macrophage (CD63, CD206, CD163) in the clinical samples (n = 4) of stomach adenocarcinoma. P values are shown as: *P < 0.05; **P < 0.01; ***P < 0.001.
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Frontiers in immunology
January 01 2022

Fig 3: Effect of EPHB2 on infiltrating immune cells of TME. (A) The distribution of immune cell clusters in UMAP plot. (B) The expression of EPHB2 in distinct clusters of immune cells. (C) Cell type fraction of each sample. (D) Correlation analysis between expression of EPHB2 in macrophages M0 and composition of infiltrating macrophages M2. Correlation analysis between expression of EPHB2 in macrophages M2 and composition of infiltrating activated NK cells (E) and resting NK cells (F). (G) Correlation network between tumor infiltrating immune cells. (H) The ligand-receptor interaction between macrophages M2 and activated NK cells. (I) The ligand-receptor interaction between macrophages M2 and resting NK cells. (J) Expression of EPHB2 and macrophages M2 markers in macrophages M0 and M2. (K) Expression of EPHB2 in normal macrophages M0, M2 and Gln-deprived macrophages M0, M2. (L) Co-localization between EPHB2 and CD206 detected by IF in LUAD specimen. “**” means that p < 0.01; "“***” means that p < 0.001; ns, no significance.
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Cell death discovery
September 24 2021

Fig 4: Exosomal lncRNA RP11-465B22.8 promotes M2 polarization.A TEM analysis of isolated exosomes from KYSE-150 cells with lncRNA RP11-465B22.8 knockdown. B The size distribution of exosomes from KYSE-150 cells with lncRNA RP11-465B22.8 silencing. C Western blot analysis of the expression of CD9 and CD61 in exosomes from lncRNA RP11-465B22.8-silenced KYSE-150 cells. D qRT-PCR analysis of lncRNA RP11-465B22.8 in exosomes from KYSE-150 cells transfected with si-P11-465B22.8. E Immunochemistry staining of CD9 expression in EC tissues. F qRT-PCR analysis of CD68 in THP-1 cells with or without PMA treatment. G Immunofluorescence staining detecting the uptake of PKH67-labeled (green) exosomes by PMA-induced macrophages. DAPI was used to stain the nucleus. H qRT-PCR of the mRNA expression of CD206, CD163, and Arginase in PMA-treated THP-1 cells. I Western blot analysis of the protein expression of CD206, CD163 in PMA-treated THP-1 cells. J Immunochemistry staining of CD163 expression in EC tissues. *P < 0.05, **P < 0.01.
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Clinical and translational medicine
February 01 2022

Fig 5: The relationship among CCCTC‐binding factor (CTCF), PTGS2 antisense NF‐κB1 complex‐mediated expression regulator RNA (PACERR) and E1A binding protein p300 (EP300) in tumour‐associated macrophages (TAMs) and prediction values of CTCF, PACERR and EP300 in pancreatic ductal adenocarcinoma (PDAC). (A) The immunofluorescence and fluorescence in situ hybridisation (FISH) analysis of CTCF (pink), EP300 (yellow), CD206 (red), PACERR (green) and DAPI (blue) in PDAC tissues. Scar bar: 20μm. (B) Staining intensity of CTCF, PACERR, EP300 and DAPI on the immunofluorescence from TAMs of 110 PDAC patients. Pink represents CTCF. Green represents PACERR. Yellow represents EP300. Blue represents DAPI. (C) The Spearman correlation between the expression of CD206 and the degree of co‐localisation of CTCF, PACERR and EP300. The rates of the triple‐positive (CTCF+‐PACERR+‐EP300+) in TAMs were used to evaluate the degree of colocalisation of CTCF, PACERR and EP300. (D) and (E) PAAD patients from TCGA were divided into high group and low group based on the median expression of the multi‐signature of CTCF, PACERR and EP300. Kaplan‐Meier curves analysis of the overall survival (OS) (D) and disease‐free survival (E) between two groups. (F) 110 PDAC patients were divided into high group and low group based on the median of rates of triple‐positive cells in TAMs (the percentage of CTCF+‐PACERR+‐EP300+ TAMs number in total CD206 positive cells number). Kaplan‐Meier curves analysis of the overall survival (OS) between two groups

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Cell Signaling Technology for
CD206/MRC1 (E6T5J) XP ® Rabbit mAb

Cell Signaling Technology's CD206/MRC1 (E6T5J) XP ® Rabbit mAb is a Rabbit monoclonal antibody. The CD206/MRC1 (E6T5J) XP ® Rabbit mAb Antibody was generated using MRC1 as the antigen. It reacts with Homo Sapiens, Human, Mouse, Rat, and Non-Human Primate. This antibody has been shown to work in applications such as: Precipitation, Simple Western, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry - fixed, Immunoprecipitation, and Western Blot.

Product Specs
ItemCD206/MRC1 (E6T5J) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $172.00 Inquire
Catalog Number24595T
ClonalityMonoclonal
ReactivityH, M, R, Mk
ApplicationsW, W-S, IP, IHC-Bond, IHC-P, IF-F, IF-IC
HostRabbit
Quantity20 µl
TargetMRC1 mouse
NCBI Full Gene Namemannose receptor, C type 1
NCBI Gene AliasesCD206, MR

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(1) Circadian disruption exacerbates MASH by reducing Akkermansia muciniphila via the FXR-CYP7A1-bile acid axisiScienceApril 17, 2026Hanxin Xue, Danyi Zeng, Yanying You, Jianwei Zhang, Xiaoning Chen, Qingqing Xing, Weiwei Zeng, Minxia Wu, Jinshui Pan, Yueyong Zhu,Cell Signaling Technology,Cat# 91882; RRID: AB_2797422 Rabbit anti-CD206 antibody,Cell Signaling Technology,Cat# 24595; RRID: AB_2892682 Mouse anti-FXR/NR1H4 antibody,Cell Signaling Technology,Cat# 72105; RRID: AB_2941911 Rabbit

(2) Nerves Stimulates Crosstalk between Gastric Cancer and Group 3 Innate Lymphoid Cells to Enhance ImmunosuppressionCancer ResearchApril 15, 2026Fangli Liao, Yanran Tong, Hua Sun, Sen Chen, Siyang Wen, Yan-e Du, Linshan Jiang, Tong Huang, Manran Liu, Weixian Chen, et al.anti-CD163 (Abcam, ab182422, RRID : AB_2753196, 1:1,000), and anti-CD206 (Cell Signaling Technology, 24595S, RRID: AB_2892682, 1:500) for M2 cells; anti-CD11b (HUABIO, HA722856, 1:2,000) for myeloid cells; anti-CD8

(3) Magnesium isoglycyrrhizinate ameliorates acetaminophen-induced acute liver injury with hyperglycaemia by modulating autophagy and oxidative stress in liver-resident macrophagesFront PharmacolApril 10, 2026Qingfa Bu, Yuan Liang, Zibo Xu, Bozhou Pan, Jian Wang, Lei Liu, Xuhao Ni, Qi Wangwith rabbit anti-mouse antibodies against iNOS (Cat# 13120T, Cell Signaling Technology) and CD206 (24595T, Cell Signaling Technology), followed by treatment with a goat anti-rabbit IgG conjugated to Texas Red

(4) Correlation of E-cadherin, vimentin, CD206, programmed cell death receptor 1, and programmed cell death ligand 1 expressions with clinicopathological factors and prognosis in oral squamous cell carcinomaJ Int Med ResApril 9, 2026Zheng-Yi Lai, Jie Ma, Jia-Yi Yin, Xiu-An Zhu, Jing-Ping Zhou, De-Tao Taomonoclonal antibodies, specifically, E-cad (3195S, 1:200 dilution), vimentin (5741S, 1:100 dilution), CD206 (24595S, 1:200 dilution), PD-1 (86163S, 1:100), and PD-L1 (13684S, 1:200). A secondary antibody was added to

(5) SARM1 is required for macrophage immunophenotype switching that is essential for nerve repairbioRxivApril 9, 2026Julianna Bennett, Halimah Adesunkanmi, Noah Leever, Grace Bergeron, Josh Small, Cathryn Holladay, Grace Saxman, Rachel E. Williamson, Manshi Swain, Gavin Pearson, et al.(Cell Signaling Technologies, #93668), α-CD14 (Cell Signaling Technologies, #93882), α-CD206 (Cell Signaling Technologies, #24595), Dectin-1 (Cell Signaling Technologies, #30260), α-GAPDH (Cell Signaling Technologies, #5174), α-iNOS

(6) Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regenerationBioact MaterApril 8, 2026Yanshuang Zhang, Tao Sun, Wenqian Li, Ye Fu, Yue Yu, Runjia Shen, Jiaqi Li, Lin Yu, Jiandong Ding(Cell Signaling Technology, 3935), HSP70 (ABclonal, A23457), NF-κB p65 (ABclonal, A19653), CD206 (Cell Signaling Technology, 24595), and FAPα (ABclonal, A23789). Following thorough washing, sections were incubated with species-matched

(7) Effects of the Dental Implant Surface Topography and Macrophage Polarisation on Osteogenesis and AngiogenesisInternational Dental JournalApril 1, 2026Bin Liu, Yanxiao Du, Mengya Wang, Zheng Zhang, Wenjie Yin, Yiming Hu, Feng Yanglisted as follows: iNOS (#20609, 1:1000 for western blot and 1:800 for immunofluorescence), CD206 (#24595, 1:1000 for western blot and 1:400 for immunofluorescence), TNF-α (#13377, 1:1000), IL-6 (#12153, 1:1000),

(8) Pauci-Immune Endocapillary Proliferative Glomerulonephritis With Glomerular M2 Macrophage InfiltrationKidney International ReportsApril 1, 2026Lei Ma, Meizi Kang, Ziyang Qiao, Shaojun Liu, Guolan Xing, Ruimin Hu, Yafen Yu, Rong Tan, Ruoyu Jia, Zhengyun Zhu, et al.marker, Abcam), CD86 (91882S, M1 macrophage marker, Cell Signaling Technology, Danvers, MA), CD206 (24595S, M2 macrophage marker, Cell Signaling Technology), CD56 (99746S, Cell Signaling Technology), CD3 (ab11089,

(9) Quantitative imaging of schwannoma captures heterogeneity and accelerates preclinical testing, revealing distinct impacts of targeted therapiesbioRxivMarch 31, 2026Emily A. Wright, Jeremie Vitte, Sara I. Veiga, Sarah E. Bushnell, Catherine E. Movsessian, Youwen Zhang, Jacquelyn Curtis, Ryan B. Corcoran, Shannon L. Stott, Marco Giovannini, et al.anti-β-catenin rabbit mAb (1:5000; clone E247, AB32572, Abcam), anti-CD206/MRC1 rabbit mAb (1:2000; clone E6T5J, 24595 Cell Signaling Technology), anti-CD11c rabbit mAb (1:2000; clone D1V9Y, 97585, Cell Signaling Technology),

(10) Systematic Pan-Cancer Characterization of ST3GAL4 Reveals Its Prognostic and Immunologic AssociationsBiomedicinesMarch 27, 2026Fushu Luo, Xiaoshun Sun, Changwu Wu, Jun Tan, Yimin Panantibodies: ST3GAL4 (human, 1:150, Proteintech, 13546-1-AP), CD206 (human, 1:400, Cell Signaling Technology, 24595S), CD68 (human, 1:500, Abcam, Cambridge, United Kingdom, ab213363), CD4 (human, 1:200, Cell Signaling

(11) The CHI3L1-neutrophil axis drives immune suppression and breast cancer metastatic disseminationJCI InsightMarch 23, 2026Tarek Taifour, Adéline Massé, Yu Gu, Virginie Sanguin-Gendreau, Dongmei Zuo, Bin Xiao, Emilie Solymoss, Yunyun Shen, Hailey Proud, Sherif Samer Attalla, et al.90120), MPO (1:500; Abcam ab208670), F4/80 (1:400; Cell Signaling 70076), CD206 (1:400; Cell Signaling 24595), p-STAT1 (1:400; Cell Signaling 9167), laminin (1:200; Abcam ab11575), collagen IV (1:100; Abcam ab6586),

(12) Etifoxine drives macrophage M2 polarization via Schwann cell-derived progesterone activation of PPARγ to accelerate peripheral nerve repairFrontiers in Cellular NeuroscienceMarch 23, 2026Chao Guo, Song Liufollowing primary antibodies: mouse anti-CD68 (1:200; Santa Cruz sc-20060), rabbit anti-CD206 (1:500; CST #24595), rabbit anti-NF200 (1:500; CST #30564), and rabbit anti-PPARγ (1:300; e.g., CST #2435). Then, the sections

(13) CX3CL1-CX3CR1 signaling orchestrates malignant progression of prostate cancer through luminal progenitor-macrophage crosstalkCell Oncol (Dordr)March 23, 2026Yu Jiang, Yuchen Guo, Lizhuang Han, Shiwei Wang, Fang Wang, Yongliang Zhao, Jiajia Wang, Junyan Han, Miaomiao Liu, Zhihua Liu, et al.were used: anti-F4/80 (1:200, Cat# 70076, Cell Signaling Technology, USA), anti-CD206 (1:300, Cat# 24595, Cell Signaling Technology, USA), anti-CX3CR1 (1:250, Cat# ab250888, Abcam, USA), anti-CX3CL1 (1:200,

(14) Kasugamycin Inhibits Melanoma Lung Metastasis and CHI3L1-Driven M2-Like Tumor-Associated Macrophage DifferentiationImmunotargets TherMarch 19, 2026Takayuki Sadanaga, Han-Seok Jeong, Roberto Cortez, Joyce H Lee, Suchitra Kamle, Bing Ma, Sung Jae Shin, Jack A Elias, Chun Geun Leeimmunoblotted with primary antibodies against CD163 (#PA5-109327, Thermo Fisher Scientific), CD206 (E6T5J, #24595S, Cell Signaling Technology), phosphorylated AKT (p-Akt) (193H12, #4058S, Cell Signaling Technology),

(15) Exosomes derived from bone marrow mesenchymal stem cells alleviate sepsis-induced ARDS via inhibition of HOXA9-mediated glycolysis in alveolar macrophagesRespiratory ResearchMarch 18, 2026Song Hu, Yu Wang, Wenyu Zhou, Lina Zhu, Yuanli Chen, Quanfu Li, Xin Lv, Hao Yangantibodies against iNOS, CD206, and F4/80 (1:500; Cell Signaling Technology, USA; Cat. Nos. 13120T, 24595T, 20395T), followed by fluorescent secondary antibody and DAPI counterstaining. Images were acquired

(16) COX5A induces M2 macrophage polarization in chronic rhinosinusitis with nasal polyps through ROS generationInflamm ResMarch 17, 2026Ji Wang, Yuan Chen, Xuemei Chen, Panhui Xiong, Bowen Zheng, Yue Gu, Dayu Guan, Yang Shen, Tao Lu, Yucheng YangG0001) and then incubated with primary antibodies against CD206(1:400,Cell Signaling Technology (CST), 24595), COX5A (1:500, ABclonal, A6437), and TOM20 (1:400, CST, 42406), followed by secondary antibody incubationThe

(17) MYC promotes the progression of prostate cancer by regulating CD47 to induce an immunosuppressive microenvironmentCell Mol Life SciMarch 11, 2026Shiyong Xin, Junjie Su, Le Zhao, Ruixin Li, Guanyu Li, Wang Qin, Zheng Zhang, Chu Wang, Yingao Zhu, Liming Feng, et al.mounting medium.(Myc: Abcam, ab32072; CD47: Proteintech, 20305-1-AP; CD206: Cell Signaling Technology, 24595 T; CD8: Abcam, ab237709). Statistical analysis All bioinformatics data were analyzed using R software

(18) Activating mutations in ESR1 contribute to an immunosuppressive breast tumor microenvironment by dampening cytokine secretionJCI InsightMarch 9, 2026Yu Gu, Dongmei Zuo, Qi-Xin Hu, Virginie Sanguin-Gendreau, Alain Pacis, Marie-Christine Guiot, Alexander Chih-Chieh Chang, Tarek Taifour, Chen Ling, Adrian V. Lee, et al.ab16669, 1:200), CD4 (CST 25229, 1:50), CD8 (CST, 98941, 1:200), F4/80 (CST, 70076, 1:200), CD206 (CST, 24595, 1:400), p-Stat1 (CST, 9167, 1:200), MHC II (BD, 107601, 1:200), Granzyme B (CST, 44153, 1:200), PD-1

(19) Wenyang Decoction Ameliorates DSS-Induced Ulcerative Colitis by Regulating Macrophage Polarization and the PI3K/AKT/mTOR/HIF-1α Signaling PathwayMediators InflammMarch 6, 2026Shengwei Li, Guanghui Yuan, Chan Chen, Jihong Lu, Xing Zhang(Catalog No. 80366 s) from CST (Denver, USA). For western blotting, antibodies for CD206 (Catalog No. 24595, dilution 1:1000), inducible nitric oxide synthase (iNOS; Catalog No. 13120, dilution 1:1000), p‐PI3K

(20) M2 Macrophage-Derived Exosomes Ameliorate BPD by Inhibiting Ferroptosis via Suppression of the ZAKα-p38 Signaling PathwayAntioxidants (Basel)March 5, 2026Yuhan Pu, Mingyue Lv, Ru Yan, Honglian Zhang, Lihui Yu, Weilai Jin, Le Zhang, Zhiwei Yu, Yahui ZhouNumber,Dilution GAPDH,Mouse,Proteintech,Cat#60004-1-Ig,"1:50,000" IL-1β,Rabbit,Novusbio,Cat#NB600-633,1:1000 IL-6,Rabbit,Novusbio,Cat#NB600-1131SS,1:1000 CD206,Rabbit,CST,Cat#24595,1:1000 Arg-1,Rabbit,Proteintech,Cat#16001-1-AP,1:5000 TSG101,Rabbit,Proteintech,Cat#28283-1-AP,1:2000 CD63,Rabbit,Proteintech,Cat#25682-1-AP,1:1000 SFTPC,Rabbit,Proteintech,Cat#10774-1-AP,1:1000 AQP5,Rabbit,Proteintech,Cat#20334-1-AP,1:1000 GPX4,Rabbit,Proteintech,Cat#67763-1-Ig,1:1000 p-P38,Rabbit,Proteintech,Cat#28796-1-AP,1:1000 ZAKα,Rabbit,Abclonal,Cat#A7371,1:1000 BCL-2,Mouse,Proteintech,Cat#68103-1-Ig,1:5000 BAX,Rabbit,Proteintech,Cat#50599-2-Ig,1:2000 Cleaved-caspase3,Mouse,Proteintech,Cat#68773-1-Ig,1:2000 p-AKT(Ser473),Mouse,Proteintech,Cat#66444-1-Ig,1:2000 The

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Molecular therapy. Nucleic acids

Frontiers in immunology

Frontiers in immunology

Cell death discovery

Clinical and translational medicine

CD206/MRC1 (E6T5J) XP ® Rabbit mAb from Cell Signaling Technology

Cell Signaling Technology

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CD206/MRC1 (E6T5J) XP ® Rabbit mAb from Cell Signaling Technology

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