Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CALCOCO2 monoclonal antibody.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CALCOCO2 (Cat# RC203843, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CALCOCO2(Cat# TA501971). Positive lysates [LY417045] (100ug) and [LC417045] (20ug) can be purchased separately from OriGene.

HEK293T cells transfected with either RC203843 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CALCOCO2 antibody (TA501971), and then analyzed by flow cytometry.

Flow cytometric Analysis of Jurkat cells, using anti-CALCOCO2 antibody (TA501971), (Red), compared to a nonspecific negative control antibody, (Blue).