HEK-293 cells transiently transfected with GRPR were grown on poly-L-lysine-coated coverslips overnight. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti-GRPR Recombinant Rabbit Monoclonal Antibody (Product # 703928, 1:500 dilution). Cells were then incubated with Alexa 488-conjugated secondary antibody for 2 h at room temperature, mounted and examined using an Immunofluorescence microscope. Note, strong staining of transfected cells.

Sections of human duodenum were dewaxed, microwaved in citric acid and incubated with rabbit monoclonal Anti-GRPR Recombinant Rabbit Monoclonal Antibody (Product # 703928, 1:500 dilution). Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Note, strong staining of neurons of mucosal plexus. Inset, peptide neutralization control.