Immunohistochemical analysis of CD61 (Integrin beta 3) performed on paraffin embedded mouse bone marrow using a CD61 (Integrin beta 3) monoclonal antibody (Product # MA5-35245) at a dilution of 1:100. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

Western blot analysis of CD61 (Integrin beta 3) in extracts of various cell lines. Samples were incubated with CD61 (Integrin beta 3) Monoclonal antibody (Product # MA5-35245) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.