Fig 1: Combined PARP and PI3K inhibition induces cell line-specific increases in proportion of TNBC cancer stem cells.Flow cytometric analyses and quantifications of CSC phenotype in TNBC cells treated with vehicle control (VC) or combined PARP (Olaparib; O) and PI3K (Buparlisib; B) inhibitors (OB) for 72 hrs (A - F). Cells were dissociated and incubated with viability dye, anti-CD44 and anti-CD24 antibodies. BT-20 cell data (A – B); HCC1937 cell data (C – D); MDA-MB-231 cell data (E – F). Flow cytometry scatter plots representative of at least 3 replicates (A, C, E). Data is mean ±SD in (B, D, F). 2-way ANOVA, * p < 0.05, ** p < 0.005, *** p < 0.0005, **** p< 0.0001.