Fig 1: In vivo synergistic antitumor immune response of CPDH-Ce6.B16F10 tumors were obtained from mice 4 days after treatment. a Immunofluorescence staining of CRT exposure and HMGB1 release in primary B16F10 tumor tissue. Scale bars: 50 μm. The experiments were repeated three times independently. b Representative flow cytometric analysis of tumor-infiltrating DCs (CD80+CD86+) and c CTLs (CD3+CD8α+), and the quantification results (right) in different groups (n = 3 independent experiments). Data are analyzed by two-sided Student’s t-test and shown as mean ± SD. d Immunofluorescence images show DC (red: CD80+, green: CD86+) and CTLs (red: CD4+ T cell, green: CD8+ T cell) infiltrated in tumors of different groups. Scale bars: 50 μm. e The corresponding quantification of the MFI of Foxp3 (n = 3 independent experiments). Data are analyzed by two-sided Student’s t-test and shown as mean ± SD. f Immunocytokine levels in sera of mice isolated 4 days after different treatments (n = 3 independent experiments). Data are analyzed by two-sided Student’s t-test and shown as mean ± SD. Source data from (b, c, e, and f) are provided as a Source Data file.