Fig 1: Multiple non‐nutritional factors control target of rapamycin (TOR) activity in developing pea embryos. RPS6‐Ser240 phosphorylation status was determined following 4 h incubations of 90–130 mg pea embryos in the light in culture media containing MS salts, 5% sucrose (w/v) and 62.5 mM Gln, with modifications as indicated. (a) Incubations with various concentrations of Gln or 2 μM AZD. (b) Timecourse incubations with or without Gln and 2 μM AZD. (c) Incubations with and without Gln in the light and dark. (d) Incubations with the auxin signaling inhibitor auxinole. (e) Incubations with the respiratory inhibitor KCN− (2 mM). (f) Incubations of older 300 mg (19 DAP) embryos with and without Gln. Replicate immunoblots are shown in Supporting Information Fig. S6. DAP, days after pollination.