Fig 1: The correlation between GINS4 methylation and clinical characteristics of HCC patients. (A) The association between GINS4 expression and its methylation level of cg26367730 methylation site in HCC samples and matched normal liver tissues from the Wanderer. Total GISN4 methylation in HCC samples was significantly lower compared with the normal liver samples evaluated by (B) UALCAN and (C) DiseaseMeth databases. The association between GINS4 methylation levels and (D) TNM stage, (E) histological grade, and (F) lymph node status of HCC patients downloaded from UALCAN database.
Fig 2: High GINS4 expression is a significantly adverse prognostic factor in HCC patients. Survival analysis shows that elevated GINS4 expressions predicts poor prognosis in (A) all HCC patients from the TCGA database, (B) HCC patients at histological grade 1, (C) HCC patients with age >60 years old, (D) HBV infection-negative HCC patients, and (E) HCC subgroup with relapse. OS, overall survival.
Fig 3: The nomogram model shows a relatively desirable predictive accuracy and clinical utility to predict OS of 371 HCC patients from the TCGA database. The time- dependent ROC demonstrated the AUC values of the nomogram, TNM stage, and GINS4 expression level at (A) 1-year, (B) 3-year, and (C) 5-year OS of HCC patients. (D) DCA for evaluating the clinical utility of nomogram model, TNM stage, and GINS4 expression level in predicting the OS of HCC cases. The x-axis revealed the range of threshold probabilities and the y-axis showed the NB. The gray line farthest left indicated all strategies. The horizontal black line represented none strategy. OS, overall survival; ROC, the receiver operating characteristic; DCA, Decision curve analysis; AUC, the area under the curve; NB, net benefit.
Fig 4: Nomogram to predict the long-term OS of 371 HCC patients from the TCGA database. (A) Nomogram model including TNM stage and GINS4 expression level to predict the 1-, 3-, and 5-year OS of HCC patients. The calibration plots of (B) 1-year, (C) 3-year, and (D) 5 year-OS of HCC patients. OS, overall survival.
Fig 5: GINS4 mRNA level is significantly correlated with multiple clinicopathologic parameters in 371 HCC cases from the TCGA database. GINS4 mRNA level in HCC patients stratified by (A) age, (B) gender, (C) AFP level, (D) residual tumor, (E) relapse, (F) histologic grade, (G) T classification, and (H) TNM stage. (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001).
Supplier Page from Abcam for Anti-SLD5 antibody