Fibronectin/FN1 (E5H6X) Rabbit mAb 26836S from Cell Signaling Technology

Product Specs
ItemFibronectin/FN1 (E5H6X) Rabbit mAb
CompanyCell Signaling Technology
Price $357.00 Inquire
Catalog Number26836S
ClonalityMonoclonal
ReactivityH
ApplicationsW, IP, IHC-Bond, IHC-P, IF-IC
Quantity100 µl
HostRabbit
Research AreaAdhesion/ECM
Molecular Weight300
TargetFN1
NCBI Full Gene Namefibronectin 1
NCBI Gene AliasesCIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF

Questions and Answers

Can this antibody be used to study fibronectin expression in pancreatic cancer cells?

Yes, the Fibronectin/FN1 (E5H6X) Rabbit mAb has been utilized in studies examining fibronectin expression in pancreatic cancer cells. For instance, it was cited in research demonstrating that MACC1 promotes pancreatic cancer metastasis by upregulating fibronectin expression, indicating its relevance in cancer biology ().

Is this antibody effective for Western blot applications?

The Fibronectin/FN1 (E5H6X) Rabbit mAb has been shown to work effectively in Western blot applications, as evidenced by its use in various studies to detect fibronectin levels in different cell types, including those involved in epithelial-mesenchymal transition processes (, ).

What is the significance of using this antibody in studies related to epithelial-mesenchymal transition (EMT)?

This antibody is significant in EMT studies as fibronectin is a key extracellular matrix protein involved in cell adhesion and migration. Research has indicated that fibronectin expression is upregulated during EMT, which is critical for cancer metastasis and progression (, ).

Does this antibody have any documented applications in immunofluorescence?

Yes, the Fibronectin/FN1 (E5H6X) Rabbit mAb has been cited in studies employing immunofluorescence to visualize fibronectin in various cellular contexts, supporting its utility in this application for studying cellular adhesion and matrix interactions ().

Cell Signaling Technology

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Sphingosine-1-Phosphate Receptor 3 Confers Tumor Metastasis in Lung Cancer Resistant to Third-Generation EGFR InhibitorMedComm (2020)April 23, 2026Mengzhen Lai, Jiaying Chen, Ye Qin, Hui Zhang, Zilu Pan, Tao Zhang, Linjiang Tong, Haotian Tang, Gang Bai, Qiupei Liu, et al.(CST, #2601S), PAK1 (CST, #2602S), ZEB1 (CST, #70512S), E‐cadherin (CST, #3195S), fibronectin (CST, #26836S), FGFR1 (CST, #9740S), STAT4 (CST, #2653S), and actin (Proteintech, 60008‐1‐Ig) at 4°C overnight and

(2) Targeting FN1 to overcome gemcitabine resistance in gallbladder cancer: Mechanistic insights and an iRGD-modified PEG-PLGA nanoparticle delivery strategyMaterials Today BioApril 1, 2026Maojin Tian, Xiangwei Meng, Weiwei Song, Zhengyu Wu, Yang Liu, Xianing Dong, Shuai Ren, Juan Du, Peiqing Zhao(rabbit; 1:1000, 4691, CST, USA), anti-p-AKT (rabbit; 1:2000, 4060, CST, USA), anti-FN1 (rabbit; 1:1000, 26836, CST, USA), and anti-β-actin (rabbit; 1:1000, ab8226, Abcam, UK), β-actin served as a loading control.

(3) Developmental reprogramming underlies chemotherapy resistance in favorable-histology Wilms tumorCell ReportsMarch 24, 2026Andrew M. Fleming, Carolyn M. Jablonowski, Hongjian Jin, Siwei Zhang, Surbhi Sona, Ha Won Lee, Karissa M. Dieseldorff Jones, Changde Cheng, Beisi Xu, Christopher L. Morton, et al.RRID:AB_2043201" Fibronectin (Western Blot; monoclonal rabbit; 1:1000 dilution),Cell Signaling Technology,"cat# 26836, RRID:AB_2924220" COL1A1 (Western Blot; monoclonal rabbit; 1:1000 dilution),Cell Signaling Technology,"cat#

(4) Pelargonidin inhibits isoproterenol induced myocardial fibrosis via regulating transforming growth factor-β/Smad2/3 signaling and Th2 cytokines in micePhysiological ResearchMarch 10, 2026Juanhua Zhao, Linping Dong, Long Yao3108), Smad3 (Cat# 9523), phospho-Smad3 (Cat# 9520), α-SMA (Cat# 19245), COL3A1 (Cat# 22734), FN1 (Cat# 26836), and β-actin (Cat# 4970) were purchased from Cell Signaling Technology, Danvers, MA, USA. HRP-conjugated

(5) ATIC Knockdown Reduces B7-H3 Expression and Oncogenic Signaling in Upper Tract Urothelial Carcinoma CellsCancer Genomics ProteomicsMarch 1, 2026Hung-Lung Ke, Wei-Chi Hsu, Yin-Lun Chang, Jhen-Hao Jhan, Yi-Yang Liu, Che-Wei Chang, Hui-Hui Lin, A-Mei Huang, Yi-Ru Wu, Hao-Lun Luo, et al.ABclonalbio, New Taipei, Taiwan, ROC), RAC2 (A1139, ABclonalbio), NT5E (A25914, ABclonalbio), Fibronectin 1 (#26836, Cell Signaling Technology), Slug (NBP2-52570, Novus), Cyclin A2 (#4656, Cell Signaling Technology),

(6) Chemomechanical remodeling of glucocorticoid-sensitive fibroblasts for multimodal synergistic therapy of skin fibrosisCell Reports MedicineFebruary 17, 2026Yifei Lu, Peng Yang, Weiming Gou, Yangping Wang, Li Ran, Lin Shi, Yiming Qin, Jingyuan Li, Ning Zhang, Dongdong Xue, et al.,Cell Signaling Technology,Cat# 14074; RRID: AB_2650491 anti-Fibronectin/FN1,Cell Signaling Technology,Cat# 26836; RRID: AB_2924220 anti-HDAC2,Abcam,Cat# ab32117; RRID: AB_732777 anti-RhoA,Cell Signaling Technology,Cat#

(7) Mast Cell Chymase and Human Lung Fibroblast Interaction: Mechanisms and Implications for AsthmaInflammationFebruary 10, 2026Gunnar Pejler, Aida Paivandy, Fabio Rabelo Melo, Venkata Sita Rama Raju Allam, Agnes Öberg, Quan Wen, Peter Bergsten, Xinran O. Zhaofor Western blot analysis:From Cell Signaling Technology (Danvers, MA, USA): Fibronectin/FN1 (1:1000, #26836), FAK (1:1000, #3285), Phospho-FAK (Tyr397) (1:1000, #8556), Syntenin-1 (1:1000, #27964), Src (1:1000,

(8) A TGFB2/TNF-induced in vitro model of proliferative vitreoretinopathy (PVR) using ARPE-19 cells confirms nicotinamide as an inhibitor of EMT and VEGFA secretionPLOS OneJanuary 13, 2026Yuqing Huang, Roland Meister, Migle Lindziute, Maximilian Binter, Jan Tode, Carsten Framme, Heiko Fuchsincubated overnight at 4°C with 1:1000 dilutions of Fibronectin 1 (FN1) rabbit mAb (Cell Signaling #26836S), COL1A1 rabbit mAb (Cell Signaling #72026S), or ZO-1 rabbit mAb (Cell Signaling #13663S) in blocking

(9) Dihydroquercetin Attenuates Silica‐Induced Pulmonary Fibrosis by Modulating the Gut Microbiota and the Serum Metabolites in MiceFood Science & NutritionJanuary 7, 2026Zunqiong Ke, Lishu Lin, Yunhong Long, Wenhui Zhang, Jianmin Guo, Leyong Yuan(Danvers, MA, USA) provided antibodies for alpha‐smooth muscle actin (α‐SMA) (#19245), Fibronectin (#26836), Collagen I (#72026), and GAPDH (#5174). Proteintech Biotechnology (Wuhan, China) provided a second

(10) Loss of a primary cilia protein ARL13B promotes TGFβ-1 induced EMT of RPE in proliferative vitreoretinopathy via increasing Smad3 expressionFront Cell Dev BiolDecember 4, 2025Wenjun Sui, Xinqi Ma, Yajun Gong, Kairui Qiu, Miner Yuan, Keli Mao, Xiaobing Qian, Jieting Zeng, Yujie Li, Bingsheng Lou, et al.were visualized. Primary antibodies: ARL13B (1:1000, 17711-1-AP, Proteintech), Fibronectin (1:1000, 26836, CST, United States), Vimentin (1:1000, 5741, CST, United States), ZO-1 (1:10000, 33-9100, Thermo, United

(11) Modeling the metastatic tumor microenvironment: a co-culture platform of lung cancer and blood vessel organoids for drug evaluationScientific ReportsNovember 24, 2025Mingyun Lee, Yeo-Joon Yoon, Ji Yeon Lee, Mina Hwang, Sewon Park, Min Hak Lee, Sang-Won Um, Byoung Chul Cho, Jinguen Rheey, Toshiro Sato, et al.(Cell signaling, 3169 S, Danvers, MA, USA; R&D systems, AF385, Minneapolis, MN, USA), Fibronectin (Cell signaling, 26836 S), Collagen type Ⅰ (Cell signaling, 72026 S), Collagen type Ⅲ (Cell signaling, 66887 S), Collagen type

(12) Succinic Acid‐Induced Macrophage Endocytosis Promotes Extracellular Vesicle‐Based Integrin Beta1 Transfer Accelerating Fibroblast Activation and Sepsis‐Associated Pulmonary FibrosisAdvanced ScienceNovember 1, 2025Wenyu Yang, Ri Tang, Yang Zhou, Jinquan Zhang, Shuya Mei, Yawen Peng, Xi Huang, Shunpeng Xing, Yuan Gao, Qiaoyi Xu, et al.antibodies, including anti‐COL1A1 (72026S, CST, USA), anti‐ITGβ1 (34971S, CST, USA), anti‐Fibronectin (26836S, CST, USA), anti‐Alix (12422‐1‐AP, Proteintech, USA), anti‐Syntenin‐1 (22399‐1‐AP, Proteintech, USA),

(13) Genetic and Functional Evidence Links Germline Biallelic Inactivating Variants in WWOX to Histological Mixed‐Type Thyroid CancerAdvanced ScienceOctober 22, 2025Xiaopeng Zhang, Jian Qi, Jialiang Wang, Zhipeng Wang, Yongguang Wang, Zongtao Hu, Ao Xu, Bo Hong, Hongzhi WangUK). Anti‐PARP (#9542), GAPDH (#2118), HA (#3724), E‐Cadherin (#3195), Vimentin (#5741), Fibronectin (#26836), Ki‐67 (#9449), and γH2AX (#9718) antibodies were purchased from Cell Signaling Technology (Danvers,

(14) HMGB1-mediated macrophage polarization to M2 phenotype promotes ureteral stricture: therapeutic potential of HMGB1 inhibitorsEuropean Journal of Medical ResearchSeptember 29, 2025Jie Zheng, Lingsong Tao, Yingqing Liu, Yang Zhang, Li Cheng, Zongyao HaoChina; FAP, 1:1000, Cell Signaling Technology, #66562S, USA; FN1, 1:1000, Cell Signaling Technology, #26836S, USA; COL1A1, 1:5000, Proteintech, #67288-1-Ig, China; TGF-β1, 1:5000, Proteintech, #81746-2-RR, China;

(15) The lipid phosphatase INPP4B controls pancreatic cancer cell migration and invasion by regulating fibronectin exocytosisJournal of Biological ChemistrySeptember 15, 2025Golam T. Saffi, Nicholas Kleine, Leonardo Salmenadilution; Cell Signaling Technologies, #14543), fibronectin (1:500 dilution; Cell Signaling Technologies, #26836 human specific, no crossreactivity to bovine FN1), or β-Actin (1:1000 dilution; Cell Signaling Technologies,

(16) Dietary addition of magnesium hydride nanoparticles: a breakthrough in combating high-fat diet-induced chronic kidney diseaseMedical Gas ResearchSeptember 1, 2025Hongtao Lu, Wanqiu Chen, Yajing Ying, Deqian Gu, Rui Li, Xiangtong Li, Jin Cheng, Xuejun Sun, Yinyin Zhang, Wenrui Liu, et al.1:100; Proteintech, Wuhan, China; Cat# 21898-1-AP) and FN (rabbit, 1:100; CST, Danvers, CO, USA; Cat# 26836). This process included embedding, sectioning, dewaxing, antigen retrieval, blocking, incubation with

(17) Large‐Scale Bioprinting of Human Epiblast‐Like Models Featuring Disc‐Shaped Morphogenesis and Gastrulation EventsAdvanced ScienceSeptember 1, 2025Yixue Luo, Liheng Luo, Ling Wang, Shanshan Yang, Hongan Ren, Shaojun Liang, Xiaoyu Wang, Yijun Su, Leqian Yu, Xiaoyue Wang, et al.anti‐YAP (1:100; CST, 14074S), mouse anti‐Ki67 (1:1000; CST, 9449S), rabbit anti‐Fibronectin (1:100; CST, 26836S), mouse anti‐Integrin β1 (1:100; abcam, ab30394) and F‐actin (1:500; abcam, ab176753 (iFluor‐488) /

(18) HOPX regulates the invasion and migration abilities of hepatocellular carcinoma by targeting SNAILSci RepAugust 13, 2025Rong Wang, Haizhou Xu, Changhuan Hu, Kun Chen, Shaowen Tang, Xiaoli Wu, Qing Zhang, Qingfeng Xiangagainst HOPX(Cat#5670), ECADHERIN-1(Cat#3195), α-CATENIN(Cat#3236), VIMENTIN(Cat#5741), FIBRONECTIN(Cat#26836), SNAIL(Cat#3879), and GAPDH(Cat#2118) (all purchased from CST). This was followed by a 2-hour incubation

(19) A New Treatment Strategy for Lung Cancer With HDAC and Wnt/ β ‐Catenin Pathway InhibitorsIUBMB LifeJuly 12, 2025Elif Erturk, Oguzhan Akgun, Yaren Yildiz, Gonca Tuna, Ferda Arisubjected to electrophoresis. Western blot steps were performed as described previously [33]. Fibronectin (#26836), Vimentin (#5741), E‐Cadherin (#3195), N‐Cadherin (#13116), Acetyl‐Histone H3 Lys9 (Ac‐H3k9, #9649),

(20) A practical and safer model of nitrogen mustard injury in corneaPLoS OneJuly 3, 2025Ana M. Sandoval-Castellanos, Yao Ke, Tiffany M. Dam, Emanual Michael Maverakis, Mark J. Mannis, Xiao-Jing Wang, Min ZhaoCell Signaling Technology, Cat No. 12282, USA) and rabbit anti-FN1 (1:100, Cell Signaling Technology, Cat No. 26836, USA)) diluted in SignalStain® Antibody Diluent (Cell Signaling Technology, USA) overnight at 4°C. On

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5/4/2022Recommended FN1 Antibody Matteo MassaroI used this antibody after inducing Epithelial Mesenchymal Transition in A549 with TGFb. I was able to accurately evaluate fibronectin amount as the dilutions and conditions suggested by the vendor ... Read Review

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Fibronectin/FN1 (E5H6X) Rabbit mAb from Cell Signaling Technology

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Fibronectin/FN1 (E5H6X) Rabbit mAb from Cell Signaling Technology

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