Fig 2: Functional experiment of five risk genes. (A-B): MTS assay was used to verify the changes in cell viability after knocking down five risk genes in TU177 and AMC—HN-8 cell lines. (C-D): Transwells assay was used to verify the changes in invasion and migration ability after knocking down five risk genes in TU177 and AMC—HN-8 cell lines. (E-F): Colony formation assay was used to verify the changes in cell proliferation after knocking down five risk genes in TU177 and AMC—HN-8 cell lines. (G-H): Western blots assay was used to verify the changes in glycolysis marker protein (PFKP,PKM2) after knocking down five risk genes in TU177 and AMC—HN-8 cell lines.