Human RUNX2/CBFA1 Antibody MAB2006 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman RUNX2/CBFA1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $459.00Supplier Page View Company Product Page
Catalog NumberMAB2006
NCBI Gene AliasesOSF2, CLCD, AML3, CBFA1, PEA2aA, CCD, CBF-alpha-1, PEBP2aA, CCD1, OSF-2
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry
Conjugate/TagUnconjugated
Clone232902
ReactivityHuman,Mouse,Transgenic Mouse
IsotypeIgG2b
TargetRUNX2/CBFA1
HostRat
Quantity100 ug (also 25 ug)
NCBI Full Gene NameRUNX family transcription factor 2
ClonalityMonoclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenE. coli-derived recombinant human RUNX2/CBFA1 isoform 2, Lys233-Tyr418, Accession # Q13950
ConcentrationLYOPH
PurityProtein A or G purified from hybridoma culture supernatant
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used for immunocytochemistry, and if so, how has it been applied in research?

Yes, the RUNX2/CBFA1 antibody has been successfully used in immunocytochemistry. For instance, in a study by Elli et al. (2022), the antibody was utilized to detect RUNX2 in cultured cells, demonstrating its effectiveness in visualizing this transcription factor in cellular contexts.

What species does this antibody react with, and how has it been validated in studies?

The RUNX2/CBFA1 antibody is reactive with both human and mouse species. It has been cited in various studies, including research by Hara et al. (2021) and Langen et al. (2017), where it was employed to investigate RUNX2 expression in mouse models and human tissues, respectively, confirming its specificity and utility in different biological systems.

What applications is this antibody suitable for, and can you provide examples of its use in specific experiments?

This antibody is suitable for applications including Western blotting, flow cytometry, immunocytochemistry, and immunohistochemistry. For example, in the study by Ramasamy et al. (2014), it was used in immunohistochemistry to analyze RUNX2 expression in bone tissue, highlighting its role in osteogenesis.

How should this antibody be stored and handled after reconstitution to maintain its stability?

After reconstitution, the RUNX2/CBFA1 antibody should be stored at -20 to -70 °C under sterile conditions to maintain stability for up to six months. It is important to avoid repeated freeze-thaw cycles and to keep it in a manual defrost freezer to ensure its integrity over time.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Calcified Cartilage-Guided Identification of Osteogenic Molecules and GeometriesACS biomaterials science & engineeringMay 13, 2024Kawaai K, Kuroda Y, Matsuo K.immunoglobulin G (IgG), and 1% bovine serum albumin (BSA)/PBS, cells were incubated with rat anti-Runx2 (MAB2006, R&D systems, 5 μg/mL) and either rabbit anti-alkaline phosphatase antibody (NBP2-67295, Novus Biologicals,

(2) Proteomic analysis reveals key differences between squamous cell carcinomas and adenocarcinomas across multiple tissuesNature communicationsJuly 18, 2022Song Q, Yang Y, Jiang D, Qin Z, Xu C, Wang H, Huang J, Chen L, Luo R, Zhang X, et al.(1:100, Cell signaling technology, catalogue No. 9349), RUNX2 antibody (1:100, R&D Systems, catalogue No. MAB2006), FOXO1 antibody (1:100, Cell signaling technology, catalogue No. 2880), PRKCE antibody (1:50, Absin,

(3) Novel Pathogenetic Variants in PTHLH and TRPS1 Genes Causing Syndromic BrachydactylyJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral ResearchMarch 1, 2022Elli FM, Mattinzoli D, Lucca C, Piu M, Maffini MA, Costanza J, Fontana L, Santaniello C, Forino C, Milani D, et al.Immunocytochemistry (IF) was performed using the rat monoclonal immunoglobulin G (IgG) RUNX2 primary antibody (MAB2006; R&D systems, Minneapolis, MN, USA) and the Alexa Fluor 546 Goat anti‐rat IgG secondary antibody, while

(4) Induction of osteogenesis by bone-targeted Notch activationeLifeFebruary 4, 2022Xu C, Dinh VV, Kruse K, Jeong HW, Watson EC, Adams S, Berkenfeld F, Stehling M, Rasouli SJ, Fan R, et al.,LifeSpan BioSciences,Cat# LS-C17044,IF (1:100) Antibody,"Anti-Runx2 (Rabbit, monoclonal)",R&D Systems,Cat# MAB2006,IF (1:50) Antibody,"Anti-Dll4 (Goat, polyclonal)",R&D Systems,Cat# AF1389,IF (1:100)WB (1:200) Antibody,"Anti-Jag1

(5) Pleiotropic Roles of NOTCH1 Signaling in the Loss of Maturational Arrest of Human Osteoarthritic ChondrocytesInternational journal of molecular sciencesNovember 5, 2021Minguzzi M, Panichi V, D'Adamo S, Cetrullo S, Cattini L, Flamigni F, Mariani E, Borzì RM.TBS with 3% normal goat serum, 2% BSA, and 0.1% Triton and left 2 h at RT: anti RUNX2 (rat monoclonal MAB2006 used at 5 µg/mL, R&D Systems) and anti-HES1 (rabbit polyclonal PA5-28802 used at 5 µg/mL, ThermoFisher

(6) Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacityGenes to cells : devoted to molecular & cellular mechanismsJuly 1, 2021Hara A, Kato K, Ishihara T, Kobayashi H, Asai N, Mii S, Shiraki Y, Miyai Y, Ando R, Mizutani Y, et al.Fluorescent Protein (RFP) (600‐901‐379, Rockland Immunochemicals, 1:500); rat monoclonal anti‐Runx2 (232902, MAB2006, R&D Systems, 1:100); goat polyclonal anti‐Sox9 (AF3075, R&D Systems, 1:200); and rabbit polyclonal

(7) Shifting osteogenesis in vascular calcificationJCI insightMay 24, 2021Yao J, Wu X, Qiao X, Zhang D, Zhang L, Ma JA, Cai X, Boström KI, Yao Y.Cell Signaling Technology, 9743, 433T, and 93115); β-catenin and Cbfa1 (all from R&D Systems, AF1329 and MAB2006); osterix (Santa Cruz Biotechnology, sc-22536); Flk1 and VE-cadherin (all from BD Biosciences, 55307

(8) Lack of Endothelial Nitric Oxide Synthase Accelerates Ectopic Calcification in Uremic Mice Fed an Adenine and High Phosphorus DietThe American journal of pathologyFebruary 1, 2021Oe Y, Mitsui S, Sato E, Shibata N, Kisu K, Sekimoto A, Miyazaki M, Sato H, Ito S, Takahashi N.

(9) Pericytes as a Source of Osteogenic Cells in Bone Fracture HealingInternational journal of molecular sciencesMarch 2, 2019Supakul S, Yao K, Ochi H, Shimada T, Hashimoto K, Sunamura S, Mabuchi Y, Tanaka M, Akazawa C, Nakamura T, et al.(Santa Cruz Biotechnology, Inc., Dallas, TX, USA, #sc-22536-R), Sost (R&D systems, #AF1589), Runx2 (R&D systems, #MAB2006), and type 1 collagen (Abcam, #ab34710) were used as primary antibodies. Stained sections were observed

(10) Osteogenic Potential of Human Umbilical Cord Mesenchymal Stem Cells on Coralline Hydroxyapatite/Calcium Carbonate MicroparticlesStem cells internationalJanuary 1, 2018Day AGE, Francis WR, Fu K, Pieper IL, Guy O, Xia Z.

(11) Collagen type XV and the 'osteogenic status'Journal of cellular and molecular medicineSeptember 1, 2017Lisignoli G, Lambertini E, Manferdini C, Gabusi E, Penolazzi L, Paolella F, Angelozzi M, Casagranda V, Piva R.

(12) Dental and periodontal phenotypes of Dlx2 overexpression in miceMolecular medicine reportsMay 1, 2017Dai J, Si J, Ouyang N, Zhang J, Wu D, Wang X, Shen G.respectively; Bioworld Technology, Inc.) or anti-runt related transcription factor 2 (Runx2; 1:100; cat. no. MAB2006; R&D Systems, Inc., Minneapolis, MN, USA) overnight at 4°C. The donkey anti-rabbit AlexaFluor 488 and

(13) The Selective Serotonin Reuptake Inhibitor Fluoxetine Directly Inhibits Osteoblast Differentiation and Mineralization During Fracture Healing in MiceJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral ResearchApril 1, 2017Bradaschia-Correa V, Josephson AM, Mehta D, Mizrahi M, Neibart SS, Liu C, Kennedy OD, Castillo AB, Egol KA, Leucht P.

(14) Cell-matrix signals specify bone endothelial cells during developmental osteogenesisNature cell biologyMarch 1, 2017Langen UH, Pitulescu ME, Kim JM, Enriquez-Gasca R, Sivaraj KK, Kusumbe AP, Singh A, Di Russo J, Bixel MG, Zhou B, et al.(LS-C17044, LifeSpan BioSciences, 1:300), rabbit anti-NG2 (AB5320, Millipore, 1:100), rat anti- Runx2 (MAB2006, R&D Systems, 1:50), rabbit anti-Calcitonin receptor (ab11042, Abcam , 1:50), rabbit anti-TRAP (sc-28204,

(15) SLC20A2 Deficiency in Mice Leads to Elevated Phosphate Levels in Cerbrospinal Fluid and Glymphatic Pathway-Associated Arteriolar Calcification, and Recapitulates Human Idiopathic Basal Ganglia CalcificationBrain pathology (Zurich, Switzerland)January 1, 2017Wallingford MC, Chia JJ, Leaf EM, Borgeia S, Chavkin NW, Sawangmake C, Marro K, Cox TC, Speer MY, Giachelli CM.used: CD13 (R&D Systems, AF2335, 5μg/mL), Collagen IV (Millipore, AB756P, 20μg/mL), RUNX2 (R&D Systems, MAB2006, 2.5μg/mL), SMA (Sigma, A2547, 60μg/mL), SLC20A1 (gift of Dr. M. Levi, University of Colorado, 1:100),

(16) Transgenic Overexpression of Tissue-Nonspecific Alkaline Phosphatase (TNAP) in Vascular Endothelium Results in Generalized Arterial CalcificationJournal of the American Heart AssociationDecember 16, 2015Savinov AY, Salehi M, Yadav MC, Radichev I, Millán JL, Savinova OV.antibody (item 711‐546‐152; Jackson ImmunoResearch). Runx2 was detected with rat monoclonal antibody (item MAB2006; R&D Systems) followed by Alexa 546–labeled anti‐rat IgG antibody (item 712‐586‐153; Jackson ImmunoResearch).

(17) GATA4 is essential for bone mineralization via ERα and TGFβ/BMP pathwaysJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral ResearchDecember 1, 2014Güemes M, Garcia AJ, Rigueur D, Runke S, Wang W, Zhao G, Mayorga VH, Atti E, Tetradis S, Péault B, et al.

(18) CKD-induced wingless/integration1 inhibitors and phosphorus cause the CKD-mineral and bone disorderJournal of the American Society of Nephrology : JASNAugust 1, 2014Fang Y, Ginsberg C, Seifert M, Agapova O, Sugatani T, Register TC, Freedman BI, Monier-Faugere MC, Malluche H, Hruska KA.(MAB26291), polyclonal anti-mouse klotho antibody (AF1819), and monoclonal anti-human/mouse RUNX2/CBFA1 antibody (MAB2006; R&D Systems, Inc.) were used in this study. RT-PCR RNA was extracted from aortas and cell cultures

(19) Coupling of angiogenesis and osteogenesis by a specific vessel subtype in boneNatureMarch 20, 2014Kusumbe AP, Ramasamy SK, Adams RH.R&D Systems, 1:100), Pecam1 (553370, BD Pharmingen, 1:100), Osterix (sc-22536-R, Santa Cruz, 1:200), Runx2 (MAB2006, R&D Systems, 1:200), α-SMA-Cy3 (C6198, Sigma, 1:100), Hif1-α (ab65979, Abcam, 1:100), Collagen type

(20) Endothelial Notch activity promotes angiogenesis and osteogenesis in boneNatureMarch 20, 2014Ramasamy SK, Kusumbe AP, Wang L, Adams RH.R&D Systems, 1:100), Pecam1 (553370, BD Pharmingen, 1:100), Osterix (sc-22536-R, Santa Cruz, 1:200), Runx2 (MAB2006, R&D Systems, 1:200), α-SMA-Cy3 (C6198, Sigma, 1:100), Hif1-α (ab65979, Abcam, 1:100), Collagen type

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RUNX2/CBFA1 Antibody

RUNX2/CBFA1 Antibody

RUNX2/CBFA1 Antibody

Human RUNX2/CBFA1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (29)

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Human RUNX2/CBFA1 Antibody from R&D Systems, a Bio-Techne Brand

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