Fig 1: Inhibition of SLPI promotes PUMA expression through Akt related signaling pathway. A Western blot displaying how inhibition of SLPI decreased phosphorylation of both Akt p-fox3a proteins, while the phosphorylation of p65 was greatly increased. Scale bar representation of the protein expressions of Akt, FoxO3 and p65, respectively (B–E). Immunofluorescence results depicted the decreased levels of p-Akt and p-fox3a and upgraded levels of p-p65 in response to SLPI inhibition (F). *p < 0.05; ***p < 0.1, and ****p < 0.5
Fig 2: Form and function of AAT, NE and SLPI in plasma and tracheal aspirates (TA) of SARS-COV-2 ARDS patients.a (i) Western blot analysis of AAT in SARS-COV-2 TAs from individual SARS-CoV-2 ARDS patients labelled P1,P2,P3,P4 and the control group of nsARDS patients labelled P1,P2,P3 demonstrates cleavage of AAT and AAT-NE complexes with significantly more cleavage of AAT seen in the SARS-CoV-2 TA samples. (ii, iii) Western blot analysis of NE in the TAs of the same individuals patients show free NE and cleaved SLPI in SARS-CoV-2 TAs in contrast to the nsARDS control group. b. FRET analysis reveals active NE in all 5 TAs from SARS-COV-2 patients on their 1st day of ICU admission and inhibition with exogenous AAT. Only 1 of the 5 nsARDS TA samples demonstrated active NE which was inhibited by exogenous AAT. Significance was tested for by paired T test; *p < 0.05.
Fig 3: siSLPI enhanced chemosensitivity of colon cancer cells to Cisplatin (200 nm). Relative viability of cell treated siSLPI, Cisplatin and Cisplatin + siSLPI, the combined treatment of both Cisplatin and suppression of SLPI exhibited the lowest cell viability (A, B). Western Blot results showing how the suppression of SLPI coupled with cisplatin treatment induced higher expression levels of PUMA and BAX proteins than that of HT29 cells treated with Cisplatin or siSLPI alone (C, D). Scale bar representation of the protein expressions of PUMA and Bax respectively (E–H). *p < 0.05; **p < 0.1, and ****p < 0.5
Fig 4: Puma can increase the level of apoptosis. Western blot results exhibited how inhibition of SLPI led to elevated expressions of both BAX protein and clavated-caspase 3 (A–C). The Tunel results confirmed the presence of significant apoptosis reaction in colon cancer cells in response to SLPI inhibition (D–G). Images were taken from three random groups, and TUNEL-positive cells were counted, and the percentage was calculated for HT29 and HCT116 cells. Tune-positive cells were found in calculated and *p < 0.05; ***p < 0.1, and ****p < 0.5
Fig 5: Exogenous SLPI could get internalized into HNSCC cells. a, b The images of HN4 cells incubated without exogenous SLPI protein. c, d The images of HN4 cells incubated with 40 μg/mL exogenous SLPI protein for 1 h. Cell nucleus was stained with DAPI (blue). Cytoskeleton was stained with phalloidine (red). SLPI was stained with FITC secondary antibody (green) (scale bar: 50 μm)
Supplier Page from Abcam for Anti-SLPI antibody [31]