Western blot was performed using Anti-ADNP Recombinant Rabbit Monoclonal Antibody (Product # 702911) and a 160 kDa band corresponding to ADNP was observed across the cell lines and tissue models tested. Whole cell extracts (30 µg lysate) of T98-G (Lane 1), U-87MG (Lane 2) and tissue extract of Mouse Liver (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:5000 dilution) using the iBright FL 1500 (Product # A44115). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).