Western blot was performed using Anti-ZNF207 Recombinant Rabbit Monoclonal Antibody (Product # 703747) and a 58 kDa band corresponding to ZNF207 was observed across cell lines and tissue tested. Modified Whole cell extract (1% SDS) (30 µg lysate) of HeLa (Lane 1), U-2 OS (Lane 2), HCT 116 (Lane 3), A549 (Lane 4), NIH/3T3 (Lane 5) and tissue extract of Mouse Lung (Lane 6) were electrophoresed using Novex® NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036) at a 1:5000 dilution. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).