Immunofluorescent analysis of GRASP65 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with GRASP65 monoclonal antibody (Product # MA5-34658), followed by DAPI (blue).

Western blot analysis of GRASP65 in Positive control: Line 1: THP-1 Line 2: SKBR3. Samples were incubated with GRASP65 monoclonal antibody (Product # MA5-34658), at a dilution of 1:500.