Western blot was performed using Anti-WDR5 Recombinant Rabbit Monoclonal Antibody (Product # 703475) and a 37 kDa band corresponding to WDR5 was observed across the cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HCT 116 (Lane 1), HT-29 (Lane 2), SW480 (Lane 3), COLO 205 (Lane 4), THP-1 (Lane 5) and PC-12 (Lane 6) were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:5000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).