Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb 5625S from Cell Signaling Technology

Product Specs
ItemCleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $448.00 Inquire
Catalog Number5625S
Quantity100 µl
ApplicationsW, W-S, IP, IHC-P, IF-IC, FC-FP
ReactivityH, M, Mk
TargetPARP1 (Asp214, 89 kD) cleaved
HostRabbit
NCBI Gene AliasesADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1
ClonalityMonoclonal
NCBI Full Gene Namepoly(ADP-ribose) polymerase 1
Research AreaApoptosis
IsotypeIgG
CloneD64E10
Molecular Weight89

Questions and Answers

Can this antibody be used to detect apoptosis in cancer cells?

Yes, the Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb has been shown to effectively detect cleaved PARP, a marker of apoptosis, in various cancer studies. For instance, it has been utilized in research involving neuroblastoma and hepatocellular carcinoma, where apoptosis was induced by different therapeutic agents (, ).

Is this antibody suitable for use in immunohistochemistry?

The Cleaved PARP antibody is applicable for immunohistochemistry (IHC), as indicated by its use in studies examining tissue samples from various cancer models. It has been cited in research that involved IHC to assess apoptosis in tumor tissues (, ).

What species does this antibody react with?

This antibody is reactive with human (Homo sapiens) and non-human primate (Macaca) samples, making it suitable for studies involving these species ().

How has this antibody been utilized in recent research?

Recent studies have employed the Cleaved PARP antibody to investigate the mechanisms of apoptosis in various cancer types, including its role in the efficacy of combination therapies in neuroblastoma and hepatocellular carcinoma models (, ).

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) SNHG family lncRNAs promote Ewing sarcoma cell fitness through intronic snoRNA-guided rRNA modificationsNAR CancerAugust 26, 2026Marcela Briones-Martin-Del-Campo, Alex G Lee, Truc Dinh, Virginie Marchand, Yuri Motorin, Marta Román, Qiuli Bi, Stanley G Leung, Max A Horlbeck, Leanne C Sayles, et al.the following primary antibodies at the indicated dilutions: cleaved PARP (Cell Signaling, Cat. No. 5625, at 1:1000), SLC3A2 (Cell Signaling, Cat. No.13180, at 1:1000), and α/β-Tubulin (Cell Signaling, Cat.

(2) A novel combinatorial therapeutic strategy using resveratrol and viral oncolysis for diffuse intrinsic pontine glioma: an in vitro study3 BiotechAugust 25, 2026Karthik Gourishetti, Isaiah Davis, Shiv Patel, Karthik Rangavajhula, Christian Jacobsen, Monique Ruschel Kanitz, Darshini Kuruppu, Deepak BhereCell Signaling Technology (Danvers, MA): AKT(#9272S), Phospho-AKT(#9271S), Caspase-9(#9508T), Cleaved PARP (#5625T), Cleaved Caspase-3 (#9664T), STAT3 (#4904T) PSTAT3 (#9145T) and Beta-Actin(#8457S) overnight at 4 °C

(3) Alantolactone inhibits T-cell lymphoma progression by suppressing CD47 expression via the PI3K/AKT and ERK signaling pathwaysApoptosisAugust 23, 2026Xiaodong Li, Ningbo Pang, Yingcong Chen, Tingting Pan, Wenwen Sun, Bingrong Chen, Fuyi Xie, Yinyu Mu, Ni Li(4370), caspase-3 (9662), phospho-H2A.X (γ-H2A.X) (60,566), cleaved caspase-3 (9664), cleaved PARP (5625), CD47(63,000), and GAPDH (5174) were purchased from Cell Signaling Technology (Massachusetts, USA).

(4) Glycolytic reprogramming and the EMT drive cervical cancer progression via the NQO1/HIF1α-SIX1 axisiScienceAugust 21, 2026Guang Zhu, Zhenggen Piao, Yuan Gao, Xianling Cong, Pengcheng Wei, Anna Han, Houkun Zhou, Zhenhua Lin, Toufeng Jin,Cell Signaling Technology,Cat# 8592; RRID: AB_10891784 Rabbit monoclonal anti-Cleaved PARP,Cell Signaling Technology,Cat# 5625; RRID: AB_10699459 Rabbit polyclonal anti-BAX,Proteintech,Cat# 50599-2-Ig; RRID: AB_2061561 Mouse

(5) Synergistic lethality of combination treatment with Trop2-directed antibody-drug conjugate sacituzumab govitecan and TRAIL agonists in triple negative breast cancerBreast Cancer ResAugust 14, 2026Yonit A. Addissie, Yoshimi E. Greer, Jayakumar Nair, Stanley Lipkowitzperformed as previously described [39]. Anti-Trop2 (cat# 90540 S), anti-PARP (#9542), anti-cleaved PARP (#5625), anti-caspase 3 (#9662), anti-cleaved caspase 3 (#9661), anti-cyclin B1 (#12231), and anti-γH2AX (#9718)

(6) Eupatilin-derived compounds enhance antitumor activity and doxorubicin efficacy in triple-negative breast cancerFront OncolAugust 12, 2026Choong-Jae Lee, Jinyoung Park, Yanghee Choi, Sung Hoon Sim, Sun-Young Kongwere used for immunoblots: anti-Caspase-3 (Cell Signaling #9662, 1:1,000), anti-PARP (Cell Signaling #5625, 1:1,000), anti-AKT (Cell Signaling #9272, 1:1,000), anti-phopho-AKT (Cell Signaling #9271, 1:1,000),

(7) HMGA1 is associated with unfavorable outcome and accelerates the aggressiveness of cholangiocarcinomaSAGE Open MedAugust 4, 2026Ratthaphong Phumphu, Saowaluk Saisomboon, Piya Prajumwong, Orawan Waenphimai, Kulthida Vaeteewoottacharn, Sopit Wongkham, Ubon Cha'on, Charupong Saengboonmee, Anucha Puapairoj, Chawalit Pairojkul, et al.Caspase 8 (#4790), Caspase 9 (#9502), Cleaved caspase 3 (#9664), Caspase 3 (#9662), Cleaved PARP (#5625) and PARP (#9542). The membranes were further incubated with HRP-conjugated secondary antibodies (#7074

(8) A stress-adaptive lipid kinase axis defines metabolic vulnerabilities in neuroendocrine prostate cancerCancer CellAugust 3, 2026Yang Zheng, Caleb Cheng, Yizhi Cao, Gabriel Cruz, Yuping Zhang, Radha Paturu, Somnath Mahapatra, Jing Hu, Rahul Mannan, Hüseyin Karabürk, et al.18799S; RRID: AB_2714181 Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb,Cell Signaling Technology,Cat# 5625S; RRID: AB_10699459 Anti-Androgen Receptor Antibody,Millipore Sigma,Cat# 06-680; RRID: AB_310214 Enolase-2 (E2H9X) XP® Rabbit mAb

(9) Sotorasib combined with 3-methyladenine for the treatment of KRAS G12C-mutant pancreatic cancer and its underlying mechanismsTranslational OncologyAugust 1, 2026Yukun Li, Mengmeng Zhou, Jingjing Pan, Haochen Su, Mengke Song, Zujiao Guan, Danmai Zhao, Xiaoxin Zhao, Yiqi Zhao, Pu Chen, et al.temperature, followed by an overnight incubation at 4 °C with primary antibodies against cleaved PARP (5625, CST, USA), cleaved caspase-3 (Servicebio, Wuhan, China), Ki-67 (Servicebio, Wuhan, China), LC3 (ab51520,

(10) ABL kinase inactivation induces transcription-replication conflicts and impairs replication fork progression in metastatic small cell lung cancerCell ReportsJuly 28, 2026Jing Jin Gu, Kevin M. Scott, Arijit Ghosh, Roberto H. Barbier, Douglas C. Rouse, Jacob P. Hoj, Michael W. Caminear, Fengqi Zhang, Boya Gao, Margaret W. Barbier, et al.1:500). Phospho-Histone H2AX (Ser139) (CST 9718, 1:1000), H2AX (CST 7631, 1:1000), cleaved PARP (CST 5625, 1:1000), cleaved caspase 3 (CST 9661, 1:500), phospho-Chk1 (S345) (CST 2341, 1:1000), phosphotyrosine

(11) CDDO-Me Overcomes Gefitinib Resistance in NSCLC by Targeting the Src/STAT3 Axis to Induce Apoptosis and PyroptosisInt J Mol SciJuly 21, 2026Tongtong Li, Weiyu Du, Ruoxian Wang, Xudong Yu, Bing Zhang, Wenjuan Wang, Jiahui Xu, Hui Cao, Dongtong Tang, Ning LiuDanvers, MA, USA, #5023, 1:1000), Cleaved PARP (Cl-PARP) (Asp214) (D64E10) (CST, Danvers, MA, USA, #5625, 1:1000), DFNA5/GSDME (HUABIO, Hangzhou, Zhejiang, China, #HA723251, 1:2000). The secondary antibodies

(12) Discovery of ferroptosis-inducing R4VP compounds for targeting aggressive cancersOncogeneJuly 21, 2026Avital Oknin-Vaisman, Deepanjan Panda, Rostislav Novak, Ghazal Kheshaiboun, Eliya Bitman-Lotan, Satish Gandhesiri, Rubina Kazi, Nikolett Pahor, Viktoria von Heyl zu Herrnsheim, Yamen Abu Ahmad, et al.antibody (# sc-376248) was from Santa Cruz Biotechnology. Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (#5625). Anti-biotin (D5A7) Rabbit Monoclonal Antibody #5597 from CST. Monoclonal Anti-PARP antibody produced in mouse

(13) Identification of FXYD5 as an oncogene in pediatric papillary thyroid canceriScienceJuly 17, 2026Ju Yu, Bo Lin, Wanna Chen, Qin Tang, Liang Zheng, Xiaoli Liang, Fang Wang, Sui Peng, Yihao Liu, Jie Li, et al.9662S; RRID: AB_331439 Rabbit anti-cleaved PARP Monoclonal Antibody,Cell Signaling Technology,Cat# 5625T; RRID: AB_10699459 Rabbit anti-cleaved Caspase-3 Monoclonal Antibody,Cell Signaling Technology,Cat#

(14) Repurposing primaquine diphosphate for imatinib-resistant chronic myeloid leukemia via targeting BCR-ABL and Wnt/β-catenin pathwayiScienceJuly 17, 2026Changqing Yin, Wenhao Liu, Chensheng Ma, Zhida Zhang, Zenghui Fang, Jiawei Cao, Guang Wu, Haihua Gu, Licai He,Cell Signaling Technology,Cat#2258 Cleaved Caspase-3,Cell Signaling Technology,Cat#9661 Cleaved PARP,Cell Signaling Technology,Cat#5625 p-GSK-3β (Ser9),Cell Signaling Technology,Cat#5558 beta-Catenin,Cell Signaling Technology,Cat#8480 c-Abl,Cell Signaling Technology

(15) Oncogenic EGFR rewires STING-TBK1 signalosomes to license DNA damage tolerance in NSCLCThe EMBO JournalJuly 10, 2026Qin Shen, Chen Mei, Yidan Chen, Qian Zhang, Qingzhe Wu, Xinyuan Yu, Fei Zhang, Shengduo Liu, Chen Chen, Cunqi Ye, et al.T Anti-pY100,Cell Signaling,Cat# 9411S Anti-PARP,Cell Signaling,Cat# 9542S Anti-cleaved PARP,Cell Signaling,Cat# 5625 Anti-K48-Ub,Cell Signaling,Cat# 8081 Anti-K63-Ub,Cell Signaling,Cat# 5621 Anti-Caspase-3,Cell Signaling,Cat#

(16) Biotinylated Diaryl Isoxazole Derivatives with Enhanced Janus Kinase 1 Inhibitory, Antiproliferative, and Apoptotic Activities in Cancer CellsACS OmegaJune 30, 2026Melike Ergüven, Hazal Beril Çatalak Yılmaz, Deniz Lengerli, Rana Acar, Servin Bagheralmoosavi, Yağmur Öykü Carus Şahin, Mehmet Gürçay, Burcu Çalışkan, Sreeparna Banerjee, Gurkan Yesiloz, et al.(Cell Signaling Technologies, Cat. No. 8438), and cleaved PARP (Cell Signaling Technologies, Cat. No. 5625) were utilized. Secondary antibodies included HRP-conjugated goat antimouse IgG (H + L) (Advansta, Cat.

(17) Convection-Enhanced Delivery of Tumor-Infiltrating Lymphocytes Enhances Intratumoral Distribution and Therapeutic Efficacy in an Orthotopic Rat Glioma ModelBiomedicinesJune 28, 2026Yuan Zhou, Liwen Zhu, Xinglei Liu, Chunxia Ji, Jiakai Yao, Di Chen, Yu Yaowith primary antibodies against cleaved PARP (Cell Signaling Technology, Danvers, MA, USA; Cat. No. 5625), cleaved caspase-3 (Cell Signaling Technology, Danvers, MA, USA; Cat. No. 9664), Ki67 (Abcam, Cambridge,

(18) Molecular Profiling and Selective Pro-Apoptotic Activity of a Pruning-Derived Lavandula dentata Leaf-Surface Extract in Colorectal Cancer CellsInt J Mol SciJune 18, 2026Shiva Pouramin Arabi, Marcello Scivicco, Valentina Parisi, Emanuele Rosa, Nunziatina De Tommasi, Ammar Bader, Vitale Del Vecchio, Nunzio Antonio Cacciola, Lorella Severinoanti-cleaved caspase-9 (#20750, Cell Signaling Technology, Danvers, MA, USA; 1:1000), and anti-cleaved PARP (#5625, Cell Signaling Technology, Danvers, MA, USA; 1:1000). β-Actin (ab8226, Abcam, Cambridge, UK; 1:5000)

(19) Combined inhibition of PLK4 and PPM1D promotes cell death in cancer cells with 17q amplificationCancer Cell InternationalJune 18, 2026Miroslav Stoyanov, Cecilia Aquino-Perez, Shreyasi Mazumder, Dominika Salcakova, Silvia Petrezselyova, Libor Macurek5A1E, 9664 S), Caspase 7 cleaved (Asp198) (clone D6H1, 8438 S), cleaved PARP (Asp214) (clone D64E10, 5625), PARP1 (clone 46D11, 9532), GAPDH (clone D16H11, 5174), KAP1-pS824 (#4127T), p53-pSer15 (82530), histone

(20) Carborane Hydrophobic Tags Drive Selective Degradation of Endogenous KRAS G12C via HSP70–Ubiquitin–Proteasome PathwayACS Bio & Med Chem AuJune 17, 2026Yujie Shao, Kazuki Miura, Hiroyuki NakamuraSanta Cruz Biotechnology, Inc., USA), anti-PARP (#9542P, Cell Signaling Technology Inc., USA), anti-cleaved PARP (#5625P, Cell Signaling Technology Inc., USA), anti-caspase 3 (#9662P, Cell Signaling Technology Inc., USA),

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1/19/2023Cleaved PARP Antibody for Western Blot Daniel MercerI used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 hours and harvested with RIPA buffer.

Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb

Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb from Cell Signaling Technology

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